Calmodulin / TRP channels
Name: Calmodulin
(Calmodulin)
Official Symbol: CALM1
provided by HGNC
Function:
Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
805 |
P62158 |
DIP-31794N |
P62158 |
MINT-4999725 |
107256 |
P62158 |
hsa:805 |
114180 |
|
|
Mouse
|
12313 |
P62204 |
DIP-31550N |
P62204 |
MINT-1855685 |
198454 |
P62204 |
mmu:12313 |
||
|
Rat
|
24242 |
P62161 |
DIP-29528N |
P62161 |
MINT-268601 |
246428 |
P62161 |
rno:24242 |
||
PPI pairs:
Biological Process:
- GO:0007202
: activation of phospholipase C activity - GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0007186 : G-protein coupled receptor signaling pathway
- GO:0006006 : glucose metabolic process
- GO:0005980 : glycogen catabolic process
- GO:0006936 : muscle contraction
- GO:0060315 : negative regulation of ryanodine-sensitive calcium-release channel activity
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0046209 : nitric oxide metabolic process
- GO:0030168 : platelet activation
- GO:0002576 : platelet degranulation
- GO:0060316 : positive regulation of ryanodine-sensitive calcium-release channel activity
- GO:0032465 : regulation of cytokinesis
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0010880 : regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- GO:0051592 : response to calcium ion
- GO:0044281 : small molecule metabolic process
- GO:0007268 : synaptic transmission
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC1 |
|
Calmodulin | Inference | Prediction | 11983166 | |||||
| TRPC2 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPC3 |
|
Calmodulin | Inference | Prediction | 11248050 | |||||
| TRPC4 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPC5 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPC6 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPC7 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPM2 |
|
Calmodulin | Inference | Prediction | 16461353 | |||||
| TRPM3 |
|
Calmodulin | Inference | Prediction | 22451665 | |||||
| TRPM4 |
|
Calmodulin | Inference | Prediction | 15590641 | |||||
| TRPV1 |
|
Calmodulin | Inference | Prediction | 12808128 | |||||
| TRPV2 |
|
Calmodulin | Inference | Prediction | 20686800 | |||||
| TRPV3 |
|
Calmodulin | Inference | Prediction | 19864432 | |||||
| TRPV4 |
|
Calmodulin | Inference | Prediction | 12724311 | |||||
| TRPV5 |
|
Calmodulin | Inference | Prediction | 15123711 | |||||
| TRPV6 |
|
Calmodulin | Inference | Prediction | 11248124 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 649-793 | E. coli | Not specified | Full-length | Not specified | 11983166 |
| TRPC1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 1-341 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Not specified | Full-length | In vitro translation | 16153633 |
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-619 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC3 |
|
Calmodulin | Fusion protein-pull down assay | Human | 1-340 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC3 |
|
Calmodulin | Fusion protein-pull down assay | Human | 742-795 | E. coli | Rat | Full-length | In vitro translation | 11248050 |
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Not used | Bovine adrenal cortex lysates | Xenopus | Full-length | In vitro translation | 11311128 | |
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-320 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Not specified | Not specified | Not specified | 11290752 |
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-331 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 701-807 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 762-975 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-321 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC6 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 828-883 | E. coli | Not specified | Full-length | In vitro translation | 12379180 |
| TRPC6 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-396 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPM3 |
|
Calmodulin | Fluorescence probe labeling | Human | 35-124 | E. coli | Not specified | Not specified | Not specified | 22451665 |
| TRPM3 |
|
Calmodulin | Fluorescence probe labeling | Human | 291-382 | E. coli | Not specified | Not specified | Not specified | 22451665 |
| TRPV1 |
|
Calmodulin | Fast performance liquid chromatography | Rat | 759-802 | E. coli | Human | Full-length | E. coli | 23109716 |
| TRPV1 |
|
Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | E. coli | Mouse | Full-length | E. coli | 23119017 |
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 14699077 |
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | C-terminus | E. coli | Bovine | Full-length | Not specified | 12808128 |
| TRPV1 |
|
Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | E. coli | Human | Full-length | E. coli | 23109716 |
| TRPV2 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | Not specified | E. coli | 20926660 |
| TRPV3 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | ARD domain | Not specified | Not determined | Not specified | In vitro translation | 20926660 |
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Not specified | Not specified | In vitro translation | 22492541 |
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 718-871 | E. coli | Not used | HEK293T lysates | 21837266 | |
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Human | C-terminus | E. coli | Not specified | Full-length | Not specified | 12724311 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-327 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 578-730 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | 584-725 | In vitro translation | Bovine | Full-length | E. coli | 11248124 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC3 |
|
Calmodulin | Co-immunoprecipitation | Mouse skeletal myotube lysates | 18215135 | |||||
| TRPC6 |
|
Calmodulin | Co-immunoprecipitation | PC12D | 15994335 | |||||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | HEK293T | Human | Full-length | Not specified | Full-length | 16461353 | |
| TRPV1 |
|
Calmodulin | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 12808128 | |
| TRPV6 |
|
Calmodulin | Co-immunoprecipitation | Xenopus oocyte | Rabbit | Full-length | Not specified | Full-length | 15123711 | |
| TRPV6 |
|
Calmodulin | Fluorescence resonance energy transfer | HEK293 | Rat | Full-length | Not specified | Full-length | 16959851 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
Calmodulin | Fluorescence probe labeling | Human | 720-749 | Not specified | Not determined | 30 nM | 11290752 | |
| TRPC1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 719-751 | Not specified | Not determined | 11983166 | ||
| TRPC1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 758-793 | Not specified | Not determined | 11983166 | ||
| TRPC1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 719-751 | Not specified | Not determined | 11290752 | ||
| TRPC2 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 905-934 | Not specified | Not determined | 10 nM | 11290752 | |
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 104-195 | Not specified | Not determined | 12601176 | ||
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 901-936 | Not specified | Not determined | 11290752 | ||
| TRPC3 |
|
Calmodulin | Fluorescence probe labeling | Human | 764-793 | Bovine | Not determined | 100 nM | 11248050 | |
| TRPC3 |
|
Calmodulin | Fluorescence probe labeling | Human | 764-793 | Not specified | Not determined | 100 nM | 11290752 | |
| TRPC3 |
|
Calmodulin | Fusion protein-pull down assay | Human | 761-795 | Not specified | Not determined | 11290752 | ||
| TRPC4 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 694-718 | Bovine | Not determined | 94 nM | 11311128 | |
| TRPC4 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 829-853 | Bovine | Not determined | 138 nM | 11311128 | |
| TRPC4 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 708-728 | Bovine | Not determined | 189 nM | 11311128 | |
| TRPC4 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 695-724 | Not specified | Not determined | 200 nM | 11290752 | |
| TRPC4 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 787-812 | Not specified | Not determined | 600 nM | 11290752 | |
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 786-848 | Not specified | Not determined | 11311128 | ||
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 688-759 | Not specified | Not determined | 11311128 | ||
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined | 15104175 | ||
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 789-812 | Not specified | Not determined | 11290752 | ||
| TRPC4 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 695-724 | Not specified | Not determined | 11290752 | ||
| TRPC5 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 702-731 | Not specified | Not determined | 220 nM | 11290752 | |
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Bovine | Not determined | 15987684 | ||
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 828-854 | Bovine | Not determined | 15987684 | ||
| TRPC5 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Not specified | Not determined | 11290752 | ||
| TRPC6 |
|
Calmodulin | Fluorescence probe labeling | 320 nM | 19932145 | |||||
| TRPC6 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 843-872 | Not specified | Not determined | 290 nM | 11290752 | |
| TRPC6 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 838-872 | Not specified | Not determined | 11290752 | ||
| TRPC7 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 769-798 | Not specified | Not determined | 19 nM | 11290752 | |
| TRPC7 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 766-798 | Not specified | Not determined | 11290752 | ||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 1-730 | Not specified | Not determined | 16461353 | ||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 406-416 | Not specified | Not determined | 16461353 | ||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 1060-1503 | Not specified | Not determined | 16461353 | ||
| TRPM3 |
|
Calmodulin | Fluorescence probe labeling | Human | 291-382 | Not specified | Not determined | 22451665 | ||
| TRPM3 |
|
Calmodulin | Fluorescence probe labeling | Human | 35-124 | Not specified | Not determined | 22451665 | ||
| TRPM4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 5-25 | Not specified | Not determined | 15590641 | ||
| TRPM4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 186-212 | Not specified | Not determined | 15590641 | ||
| TRPM4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 1076-1098 | Not specified | Not determined | 15590641 | ||
| TRPM4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 1146-1167 | Not specified | Not determined | 15590641 | ||
| TRPM4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 1095-1151 | Not specified | Not determined | 15590641 | ||
| TRPV1 |
|
Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | Mouse | Not determined | 23119017 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 189-222 | Not specified | Not determined | 14699077 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Human | 101-364 | Not specified | Not determined | 19864432 | ||
| TRPV1 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 767-801 | Bovine | Not determined | 12808128 | ||
| TRPV1 |
|
Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | Human | 11-145 | 23109716 | ||
| TRPV2 |
|
Calmodulin | Fluorescence probe labeling | Human | 654-683 | Not specified | Not determined | 191 nM | 20686800 | |
| TRPV2 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | 20926660 | ||
| TRPV3 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV3 |
|
Calmodulin | Fusion protein-pull down assay | Human | 115-367 | Not specified | Not determined | 19864432 | ||
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Chicken | 132-383 | Not specified | Not determined | 19864432 | ||
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 718-871 | Human | Not determined | 21837266 | ||
| TRPV4 |
|
Calmodulin | Fusion protein-pull down assay | Human | 812-831 | Not specified | Not determined | 12724311 | ||
| TRPV5 |
|
Calmodulin | Fluorescence probe labeling | Human | 587-616 | Not specified | Not determined | 306 nM | 20686800 | |
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV5 |
|
Calmodulin | Fusion protein-pull down assay | Human | 696-729 | Not specified | Not determined | 21576356 | ||
| TRPV5 |
|
Calmodulin | Native-gel electrophoresis | Human | 696-729 | Not specified | Not determined | 21576356 | ||
| TRPV6 |
|
Calmodulin | Fluorescence probe labeling | 1:1 | 43 nM | 12765696 | ||||
| TRPV6 |
|
Calmodulin | Fluorescence probe labeling | 65 nM | 11248124 | |||||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 327-577 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 88-97 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 643-656 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | 691-711 | Bovine | Not determined | 11248124 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC1 |
|
Calmodulin | Calcium measurement | Inhibition | 11983166 | |||||||
| TRPC1 |
|
Calmodulin | Patch clamp | Inhibition | 11983166 | |||||||
| TRPC3 |
|
Calmodulin | Patch clamp | Inhibition | 11248050 | |||||||
| TRPC6 |
|
Calmodulin | Calcium measurement | Activation | 12379180 | |||||||
| TRPC6 |
|
Calmodulin | Patch clamp | Activation | 12379180 | |||||||
| TRPM2 |
|
Calmodulin | Calcium measurement | Activation | 16461353 | |||||||
| TRPM4 |
|
Calmodulin | Patch clamp | Activation | 15590641 | |||||||
| TRPV1 |
|
Calmodulin | Patch clamp | Inhibition | 14699077 | |||||||
| TRPV1 |
|
Calmodulin | Patch clamp | Sensitization | 23109716 | |||||||
| TRPV1 |
|
Calmodulin | Patch clamp | Inhibition | 12808128 | |||||||
| TRPV4 |
|
Calmodulin | Patch clamp | Activation | 12724311 | |||||||
| TRPV5 |
|
Calmodulin | Calcium measurement | Inhibition | 21576356 | |||||||
| TRPV5 |
|
Calmodulin | Patch clamp | Inhibition | 21576356 | |||||||
| TRPV6 |
|
Calmodulin | Calcium measurement | Inhibition | 16959851 | |||||||
| TRPV6 |
|
Calmodulin | Patch clamp | Inhibition | 15123711 | |||||||
| TRPV6 |
|
Calmodulin | Patch clamp | Inhibition | 11248124 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)