Caveolin-1 / TRP channels
Name: Caveolin-1
(Caveolin-1)
Official Symbol: CAV1
provided by HGNC
Function:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
857 |
Q03135 |
DIP-5960N |
Q03135 |
MINT-152933 |
107305 |
Q03135 |
hsa:857 |
601047 |
|
|
Mouse
|
12389 |
P49817 |
DIP-35140N |
P49817 |
MINT-1542313 |
198514 |
P49817 |
mmu:12389 |
||
|
Rat
|
25404 |
P41350 |
DIP-35143N |
P41350 |
MINT-202594 |
247439 |
P41350 |
rno:25404 |
||
Biological Process:
- GO:0097190
: apoptotic signaling pathway - GO:0007596 : blood coagulation
- GO:0006816 : calcium ion transport
- GO:0070836 : caveola assembly
- GO:0072584 : caveolin-mediated endocytosis
- GO:0071455 : cellular response to hyperoxia
- GO:0009267 : cellular response to starvation
- GO:0033344 : cholesterol efflux
- GO:0042632 : cholesterol homeostasis
- GO:0030301 : cholesterol transport
- GO:0051480 : cytosolic calcium ion homeostasis
- GO:0000188 : inactivation of MAPK activity
- GO:0007595 : lactation
- GO:0050900 : leukocyte migration
- GO:0019915 : lipid storage
- GO:0032507 : maintenance of protein location in cell
- GO:0060056 : mammary gland involution
- GO:0000165 : MAPK cascade
- GO:0051899 : membrane depolarization
- GO:0046785 : microtubule polymerization
- GO:2000811 : negative regulation of anoikis
- GO:0030514 : negative regulation of BMP signaling pathway
- GO:0090090 : negative regulation of canonical Wnt receptor signaling pathway
- GO:0001960 : negative regulation of cytokine-mediated signaling pathway
- GO:0001937 : negative regulation of endothelial cell proliferation
- GO:0030857 : negative regulation of epithelial cell differentiation
- GO:0046426 : negative regulation of JAK-STAT cascade
- GO:0045019 : negative regulation of nitric oxide biosynthetic process
- GO:0033137 : negative regulation of peptidyl-serine phosphorylation
- GO:0032091 : negative regulation of protein binding
- GO:0031397 : negative regulation of protein ubiquitination
- GO:0000122 : negative regulation of transcription from RNA polymerase II promoter
- GO:0042524 : negative regulation of tyrosine phosphorylation of Stat5 protein
- GO:0033484 : nitric oxide homeostasis
- GO:0046209 : nitric oxide metabolic process
- GO:0010524 : positive regulation of calcium ion transport into cytosol
- GO:0090263 : positive regulation of canonical Wnt receptor signaling pathway
- GO:0045807 : positive regulation of endocytosis
- GO:2001238 : positive regulation of extrinsic apoptotic signaling pathway
- GO:2001244 : positive regulation of intrinsic apoptotic signaling pathway
- GO:0048554 : positive regulation of metalloenzyme activity
- GO:0031116 : positive regulation of microtubule polymerization
- GO:0033138 : positive regulation of peptidyl-serine phosphorylation
- GO:0045907 : positive regulation of vasoconstriction
- GO:0051260 : protein homooligomerization
- GO:2000286 : receptor internalization involved in canonical Wnt receptor signaling pathway
- GO:0030193 : regulation of blood coagulation
- GO:0019217 : regulation of fatty acid metabolic process
- GO:0050999 : regulation of nitric-oxide synthase activity
- GO:0006940 : regulation of smooth muscle contraction
- GO:0051592 : response to calcium ion
- GO:0042493 : response to drug
- GO:0043627 : response to estrogen stimulus
- GO:0010332 : response to gamma radiation
- GO:0051384 : response to glucocorticoid stimulus
- GO:0001666 : response to hypoxia
- GO:0009612 : response to mechanical stimulus
- GO:0007584 : response to nutrient
- GO:0032570 : response to progesterone stimulus
- GO:0007519 : skeletal muscle tissue development
- GO:0044281 : small molecule metabolic process
- GO:0031295 : T cell costimulation
- GO:0006641 : triglyceride metabolic process
- GO:0001570 : vasculogenesis
- GO:0042310 : vasoconstriction
- GO:0016050 : vesicle organization
- GO:0019048 : virus-host interaction
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
- OMIM:601047
:
Lipodystrophy, congenital generalized, type 3
(OMIM:612526 )
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC1 |
|
Caveolin-1 | Inference | Prediction | 10766822 | |||||
| TRPC3 |
|
Caveolin-1 | Inference | Prediction | 11524429 | |||||
| TRPC4 |
|
Caveolin-1 | Inference | Prediction | 17416589 | |||||
| TRPV4 |
|
Caveolin-1 | Inference | Prediction | 20705915 | |||||
(:
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
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| Validation: In vitro validation
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC1 |
|
Caveolin-1 | Fusion protein-pull down assay | Human | 1-347 | E. coli | Not specified | Full-length | HSG cell lysates | 12732636 |
| TRPC1 |
|
Caveolin-1 | Yeast two-hybrid | Not specified | 317-347 | Yeast | Not specified | Full-length | Yeast | 19897728 |
| TRPC4 |
|
Caveolin-1 | Fusion protein-pull down assay | Not used | Mouse lung endothelium cell lysates | Not specified | Full-length | E. coli | 17416589 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | HUVEC | Human | Full-length | Mouse | Full-length | 20705915 | |
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | Rat caudal artery section | 14551243 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | HSG cell | Not used | Not specified | Full-length | 12732636 | ||
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | HSG cell | Human | Full-length | Not specified | Full-length | 12732636 | |
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | MDCK | Human | Full-length | Not specified | Full-length | 12732636 | |
| TRPC1 |
|
Caveolin-1 | Co-immunofluorescence staining | HSG cell | 19897728 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | C2C12 | 19351713 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | Rat caudal artery cell | 14551243 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | HSG cell | 18430726 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | HSG cell | Human | Full-length | Not used | 10766822 | ||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | MDCK | Human | Full-length | Not specified | Full-length | 12732636 | |
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | HSG cell | 19897728 | |||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | HSG cell | Human | Full-length | Not used | 10980191 | ||
| TRPC1 |
|
Caveolin-1 | Fluorescence resonance energy transfer | HSG cell | Not specified | Full-length | Not specified | Full-length | 19897728 | |
| TRPC3 |
|
Caveolin-1 | Co-immunofluorescence staining | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 |
|
Caveolin-1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC4 |
|
Caveolin-1 | Co-immunofluorescence staining | Mouse lung endothelium cell | 17416589 | |||||
| TRPC4 |
|
Caveolin-1 | Co-immunoprecipitation | Mouse lung endothelium cell | 17416589 | |||||
| TRPV4 |
|
Caveolin-1 | Co-immunofluorescence staining | HUVEC | Human | Full-length | Mouse | Full-length | 20705915 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | Human | 781-789 | Human | Not determined | 16822931 | ||
| TRPC1 |
|
Caveolin-1 | Co-immunoprecipitation | Human | 781-789 | Human | 82-101 | 19052258 | ||
| TRPC1 |
|
Caveolin-1 | Fusion protein-pull down assay | Human | Not determined | Human | 82-101 | 16822931 | ||
| TRPC1 |
|
Caveolin-1 | Yeast two-hybrid | Human | 271-349 | Not specified | Not determined | 12732636 | ||
| TRPC4 |
|
Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 61-101 | 17416589 | ||
| TRPC4 |
|
Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 135-178 | 17416589 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC1 |
|
Caveolin-1 | Cell surface biotinylation | Increase in plasma membrane level | 19897728 | |||||||
(:
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:
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