TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

D2R / TRP channels

Basic Information
External Database
PPI pairs (3)
Biological Process (73)
Disease (1)

Name: D(2) dopamine receptor (D2R)

Official Symbol: DRD2 provided by HGNC

Function:

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (By similarity).

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

Biological Process:

GO:0032147 : activation of protein kinase activity
GO:0021984 : adenohypophysis development
GO:0007195 : adenylate cyclase-inhibiting dopamine receptor signaling pathway
GO:0007628 : adult walking behavior
GO:0050482 : arachidonic acid secretion
GO:0031223 : auditory behavior
GO:0007409 : axonogenesis
GO:0048148 : behavioral response to cocaine
GO:0048149 : behavioral response to ethanol
GO:0048755 : branching morphogenesis of a nerve
GO:0021853 : cerebral cortex GABAergic interneuron migration
GO:0032922 : circadian regulation of gene expression
GO:0042417 : dopamine metabolic process
GO:0051482 : elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0007631 : feeding behavior
GO:0002031 : G-protein coupled receptor internalization
GO:0007625 : grooming behavior
GO:0007243 : intracellular protein kinase cascade
GO:0007616 : long-term memory
GO:0007194 : negative regulation of adenylate cyclase activity
GO:0045776 : negative regulation of blood pressure
GO:0030336 : negative regulation of cell migration
GO:0008285 : negative regulation of cell proliferation
GO:0042321 : negative regulation of circadian sleep/wake cycle, sleep
GO:0060160 : negative regulation of dopamine receptor signaling pathway
GO:0046676 : negative regulation of insulin secretion
GO:0051898 : negative regulation of protein kinase B signaling cascade
GO:0050709 : negative regulation of protein secretion
GO:0051967 : negative regulation of synaptic transmission, glutamatergic
GO:1901386 : negative regulation of voltage-gated calcium channel activity
GO:0001976 : neurological system process involved in regulation of systemic arterial blood pressure
GO:0007270 : neuron-neuron synaptic transmission
GO:0030432 : peristalsis
GO:0046488 : phosphatidylinositol metabolic process
GO:0060158 : phospholipase C-activating dopamine receptor signaling pathway
GO:0043473 : pigmentation
GO:0051586 : positive regulation of dopamine uptake involved in synaptic transmission
GO:0070374 : positive regulation of ERK1 and ERK2 cascade
GO:0045745 : positive regulation of G-protein coupled receptor protein signaling pathway
GO:0060124 : positive regulation of growth hormone secretion
GO:0040018 : positive regulation of multicellular organism growth
GO:0002052 : positive regulation of neuroblast proliferation
GO:0002092 : positive regulation of receptor internalization
GO:0035815 : positive regulation of renal sodium excretion
GO:0045944 : positive regulation of transcription from RNA polymerase II promoter
GO:0035810 : positive regulation of urine volume
GO:0060134 : prepulse inhibition
GO:0008104 : protein localization
GO:0051481 : reduction of cytosolic calcium ion concentration
GO:0030814 : regulation of cAMP metabolic process
GO:0014059 : regulation of dopamine secretion
GO:0002027 : regulation of heart rate
GO:0048169 : regulation of long-term neuronal synaptic plasticity
GO:0043666 : regulation of phosphoprotein phosphatase activity
GO:0043266 : regulation of potassium ion transport
GO:0002028 : regulation of sodium ion transport
GO:0032228 : regulation of synaptic transmission, GABAergic
GO:0051209 : release of sequestered calcium ion into cytosol
GO:0001975 : response to amphetamine
GO:0048678 : response to axon injury
GO:0042493 : response to drug
GO:0034776 : response to histamine
GO:0001666 : response to hypoxia
GO:0010039 : response to iron ion
GO:0043278 : response to morphine
GO:0035094 : response to nicotine
GO:0009636 : response to toxin
GO:0007608 : sensory perception of smell
GO:0007416 : synapse assembly
GO:0001963 : synaptic transmission, dopaminergic
GO:0001659 : temperature homeostasis
GO:0008542 : visual learning
GO:0016055 : Wnt receptor signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease:

OMIM:126450 : Dystonia, myoclonic (OMIM:159900 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC1	D2R	Yeast two-hybrid	Not used as a bait		Human	Fetal brain	cDNA library		18261457
TRPC4	D2R	Inference						Prediction	18261457
TRPC5	D2R	Inference						Prediction	18261457

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC1	D2R	Fusion protein-pull down assay	Human	638-759	E. coli	Human	131-151	E. coli	18261457
TRPC4	D2R	Fusion protein-pull down assay	Human	621-893	E. coli	Human	131-151	E. coli	18261457
TRPC5	D2R	Fusion protein-pull down assay	Human	619-973	E. coli	Human	131-151	E. coli	18261457

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC1	D2R	Co-immunofluorescence staining	Monkey prefrontal cortex						18261457
TRPC1	D2R	Co-immunoprecipitation	Rat striatal tissue						18261457
TRPC1	D2R	Co-immunoprecipitation	Rat cortex tissue						18261457

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC1	D2R	Yeast two-hybrid	Human	726-745	Human	131-151			18261457
TRPC4	D2R	Yeast two-hybrid	Human	712-731	Human	131-151			18261457
TRPC5	D2R	Yeast two-hybrid	Human	719-738	Human	131-151			18261457

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel		Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC1		D2R	Cell surface biotinylation		Increase in plasma membrane level		18261457

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

D2R / TRP channels