IP3R1 / TRP channels
Name: Inositol 1,4,5-trisphosphate receptor type 1
(IP3R1)
Official Symbol: ITPR1
provided by HGNC
Function:
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
3708 |
Q14643 |
DIP-29714N |
Q14643 |
MINT-4991320 |
109913 |
Q14643 |
hsa:3708 |
147265 |
|
|
Mouse
|
16438 |
P11881 |
DIP-32243N |
P11881 |
MINT-4099099 |
200847 |
P11881 |
mmu:16438 |
||
|
Rat
|
25262 |
P29994 |
DIP-29715N |
MINT-5026585 |
247302 |
P29994 |
rno:25262 |
|||
Biological Process:
- GO:0007202
: activation of phospholipase C activity - GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0070059 : intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0050796 : regulation of insulin secretion
- GO:0051209 : release of sequestered calcium ion into cytosol
- GO:0001666 : response to hypoxia
- GO:0044281 : small molecule metabolic process
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
- OMIM:147265
:
Spinocerebellar ataxia 15
(OMIM:606658 )
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC3 |
|
IP3R1 | Affinity purification-mass spectrometry | Not used as a bait | Rat | Brain | Crude membranes | 18205297 | ||
| TRPC3 |
|
IP3R1 | Inference | Prediction | 10518223 | |||||
| TRPC4 |
|
IP3R1 | Inference | Prediction | 11163362 | |||||
| TRPP1 |
|
IP3R1 | Affinity purification-mass spectrometry | Mouse | Not specified | Mouse | Vascular smooth muscle | MOVAS lysates | 19879844 | |
| TRPP1 |
|
IP3R1 | Inference | Prediction | 16223735 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC4 |
|
IP3R1 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2520-2670 | Yeast | 11163362 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | Rat cerebral arterial lysates | 20378853 | |||||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | Rat mesenteric artery lysates | 23045459 | |||||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | Rat brain lysates | 18205297 | |||||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Rat | Full-length | 10518223 | |
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | MDCK | Not specified | Full-length | Not used | 15623527 | ||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | HEK293 | 20022948 | |||||
| TRPP1 |
|
IP3R1 | Co-immunofluorescence staining | LLC-PK1 | Human | Full-length | Not used | 20375013 | ||
| TRPP1 |
|
IP3R1 | Co-immunoprecipitation | COS-7 | Human | Full-length | Not used | 16223735 | ||
| TRPP1 |
|
IP3R1 | Co-immunoprecipitation | Xenopus oocyte | Human | 1-741 | Not used | 16223735 | ||
| TRPP1 |
|
IP3R1 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Not used | 16223735 | ||
| TRPP1 |
|
IP3R1 | Co-immunoprecipitation | LLC-PK1 | Human | Full-length | Not used | 20375013 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC3 |
|
IP3R1 | Co-immunoprecipitation | Human | Not determined | Rat | IP3 binding domain | 10518223 | ||
| TRPC3 |
|
IP3R1 | Fusion protein-pull down assay | Mouse | 725-848 | Not specified | 654-688 | 11290752 | ||
| TRPP1 |
|
IP3R1 | Fusion protein-pull down assay | Human | Not determined | Human | 1-345 | 20375013 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC3 |
|
IP3R1 | Calcium measurement | Activation | 10518223 | |||||||
| TRPC3 |
|
IP3R1 | Patch clamp | Activation | 10518223 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)