IP3R2 / TRP channels
Name: Inositol 1,4,5-trisphosphate receptor type 2
(IP3R2)
Official Symbol: ITPR2
provided by HGNC
Function:
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
3709 |
Q14571 |
DIP-42028N |
Q14571 |
MINT-157881 |
109914 |
Q14571 |
hsa:3709 |
600144 |
|
|
Mouse
|
16439 |
Q9Z329 |
UNK-5562109 |
200848 |
Q9Z329 |
mmu:16439 |
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|
Rat
|
81678 |
P29995 |
UNK-6030828 |
249575 |
P29995 |
rno:81678 |
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Biological Process:
- GO:0007202
: activation of phospholipase C activity - GO:0071320 : cellular response to cAMP
- GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0050796 : regulation of insulin secretion
- GO:0001666 : response to hypoxia
- GO:0044281 : small molecule metabolic process
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC1 |
|
IP3R2 | Inference | Prediction | 10970773 | |||||
| TRPC2 |
|
IP3R2 | Inference | Prediction | 15329338 | |||||
| TRPC3 |
|
IP3R2 | Inference | Prediction | 11290752 | |||||
| TRPC4 |
|
IP3R2 | Inference | Prediction | 11163362 | |||||
| TRPC6 |
|
IP3R2 | Inference | Prediction | 18191041 | |||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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| Validation: In vitro validation
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC4 |
|
IP3R2 | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Rat | 2544-2701 | E. coli | 11163362 |
| TRPC4 |
|
IP3R2 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2544-2701 | Yeast | 11163362 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 11336651 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 18068335 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 16870612 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 18191041 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Rat astrocyte | 16905188 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 18249094 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 15121806 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 12196544 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 23228564 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | MEG-01 | 23228564 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 10970773 | |||||
| TRPC1 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 17681754 | |||||
| TRPC2 |
|
IP3R2 | Co-immunofluorescence staining | Mouse splenic erythroblast | 15329338 | |||||
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338 | |
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247 | |
| TRPC3 |
|
IP3R2 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 |
|
IP3R2 | Co-immunoprecipitation | Sheep uterine arterial endothelial cell | 16899571 | |||||
| TRPC6 |
|
IP3R2 | Co-immunoprecipitation | Human platelets | 18191041 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | Mouse | 1001-1036 | Rat | Not determined | 15329338 | ||
| TRPC3 |
|
IP3R2 | Fusion protein-pull down assay | Mouse | 725-848 | Not specified | 675-695 | 11290752 | ||
| TRPC4 |
|
IP3R2 | Fusion protein-pull down assay | Human | 785-868 | Rat | 2556-2713 | 11163362 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC1 |
|
IP3R2 | Calcium measurement | Activation | 12196544 | |||||||
| TRPC1 |
|
IP3R2 | Patch clamp | Activation | 12196544 | |||||||
(:
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:
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