IP3R3 / TRP channels
Name: Inositol 1,4,5-trisphosphate receptor type 3
(IP3R3)
Official Symbol: ITPR3
provided by HGNC
Function:
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
3710 |
Q14573 |
DIP-33130N |
Q14573 |
MINT-4991384 |
109915 |
Q14573 |
hsa:3710 |
147267 |
|
|
Mouse
|
16440 |
P70227 |
UNK-5382532 |
200849 |
P70227 |
mmu:16440 |
||||
|
Rat
|
25679 |
Q63269 |
UNK-5464541 |
247708 |
Q63269 |
rno:25679 |
||||
Biological Process:
- GO:0007202
: activation of phospholipase C activity - GO:0060402 : calcium ion transport into cytosol
- GO:0006112 : energy reserve metabolic process
- GO:0007173 : epidermal growth factor receptor signaling pathway
- GO:0008543 : fibroblast growth factor receptor signaling pathway
- GO:0007186 : G-protein coupled receptor signaling pathway
- GO:0048011 : nerve growth factor receptor signaling pathway
- GO:0030168 : platelet activation
- GO:0051291 : protein heterooligomerization
- GO:0051260 : protein homooligomerization
- GO:0050796 : regulation of insulin secretion
- GO:0051592 : response to calcium ion
- GO:0050913 : sensory perception of bitter taste
- GO:0050916 : sensory perception of sweet taste
- GO:0050917 : sensory perception of umami taste
- GO:0044281 : small molecule metabolic process
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC1 |
|
IP3R3 | Inference | Prediction | 10766822 | |||||
| TRPC2 |
|
IP3R3 | Inference | Prediction | 11290752 | |||||
| TRPC3 |
|
IP3R3 | Inference | Prediction | 10611319 | |||||
| TRPC4 |
|
IP3R3 | Inference | Prediction | 11290752 | |||||
| TRPC5 |
|
IP3R3 | Inference | Prediction | 11290752 | |||||
| TRPC6 |
|
IP3R3 | Inference | Prediction | 10611319 | |||||
| TRPC7 |
|
IP3R3 | Inference | Prediction | 11290752 | |||||
| TRPP1 |
|
IP3R3 | Affinity purification-mass spectrometry | Mouse | Not specified | Mouse | Vascular smooth muscle | MOVAS lysates | 19879844 | |
| TRPV4 |
|
IP3R3 | Inference | Prediction | 18378772 | |||||
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Human | 676-848 | In vitro translation | Human | 638-926 | E. coli | 10611319 |
| TRPC4 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Human | 669-698 | E. coli | 11290752 |
| TRPC4 |
|
IP3R3 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2556-2713 | Yeast | 11163362 |
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
IP3R3 | Co-immunofluorescence staining | Human keratinocyte | 15654973 | |||||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 14505576 | ||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | HSG cell | Human | Full-length | Not used | 10766822 | ||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | HSG cell | Human | Full-length | Not used | 10980191 | ||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | Human keratinocyte | 15654973 | |||||
| TRPC2 |
|
IP3R3 | Co-immunofluorescence staining | Rat vomeronasal organ | 12472899 | |||||
| TRPC2 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
| TRPC2 |
|
IP3R3 | Co-immunoprecipitation | Rat vomeronasal organ | 12472899 | |||||
| TRPC3 |
|
IP3R3 | Co-immunofluorescence staining | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 |
|
IP3R3 | Co-immunofluorescence staining | Mouse pancreatic acini | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunofluorescence staining | Mouse parotid acini | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunofluorescence staining | Mouse submandibular gland duct | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 14505576 | ||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | Mouse brain tissue | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | Mouse pancreatic tissue | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | Mouse submandibular gland lysates | 16887806 | |||||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 10611319 | |
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | MDCK | Not specified | Full-length | Not used | 15623527 | ||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 10983988 | ||
| TRPC4 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
| TRPC5 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
| TRPC6 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 14505576 | ||
| TRPC6 |
|
IP3R3 | Co-immunoprecipitation | HEK293T | Mouse | Full-length | Human | Full-length | 10611319 | |
| TRPP1 |
|
IP3R3 | Co-immunofluorescence staining | LLC-PK1 | Human | Full-length | Not used | 20375013 | ||
| TRPP1 |
|
IP3R3 | Co-immunoprecipitation | LLC-PK1 | Human | Full-length | Not used | 20375013 | ||
| TRPV4 |
|
IP3R3 | Co-immunoprecipitation | HeLa | Human | Full-length | Rat | Full-length | 18378772 | |
| TRPV4 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Human | Full-length | Rat | Full-length | 18826956 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | Human | 645-648 | Human | Not determined | 14505576 | ||
| TRPC1 |
|
IP3R3 | Co-immunoprecipitation | Human | 781-789 | Human | Not determined | 19052258 | ||
| TRPC1 |
|
IP3R3 | Fusion protein-pull down assay | Human | 719-751 | Human | 669-698 | 11290752 | ||
| TRPC2 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 901-936 | Human | 669-698 | 11290752 | ||
| TRPC3 |
|
IP3R3 | Co-immunoprecipitation | Human | 775-787 | Rat | Not determined | 18276585 | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Human | 777-797 | Human | 669-698 | 10611319 | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Human | 777-797 | Human | 751-821 | 10611319 | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Human | 761-795 | Human | 669-698 | 11248050 | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Human | 761-795 | Human | 669-698 | 11290752 | ||
| TRPC3 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 725-848 | Human | 681-698 | 11290752 | ||
| TRPC4 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 | 15104175 | ||
| TRPC4 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 781-864 | Human | 669-698 | 11290752 | ||
| TRPC4 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 | 11290752 | ||
| TRPC5 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 701-733 | Human | 669-698 | 11290752 | ||
| TRPC6 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 838-872 | Human | 669-698 | 11290752 | ||
| TRPC7 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 766-798 | Human | 669-698 | 11290752 | ||
| TRPV4 |
|
IP3R3 | Co-immunoprecipitation | Human | 812-831 | Rat | Not determined | 18826956 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC3 |
|
IP3R3 | Calcium measurement | Activation | 10611319 | |||||||
| TRPC3 |
|
IP3R3 | Calcium measurement | Inhibition | 10611319 | |||||||
| TRPC3 |
|
IP3R3 | Patch clamp | Activation | 11248050 | |||||||
| TRPV4 |
|
IP3R3 | Calcium measurement | Activation | 18378772 | |||||||
| TRPV4 |
|
IP3R3 | Calcium measurement | Activation | 18826956 | |||||||
| TRPV4 |
|
IP3R3 | Patch clamp | Activation | 18378772 | |||||||
| TRPV4 |
|
IP3R3 | Patch clamp | Activation | 18826956 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)