TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

NHERF1 / TRP channels

Basic Information
External Database
PPI pairs (2)
Biological Process (24)
Disease (1)

Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF1)

Official Symbol: SLC9A3R1 provided by HGNC

Function:

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

Biological Process:

GO:0030036 : actin cytoskeleton organization
GO:0007191 : adenylate cyclase-activating dopamine receptor signaling pathway
GO:0006915 : apoptotic process
GO:0032782 : bile acid secretion
GO:0030643 : cellular phosphate ion homeostasis
GO:0034613 : cellular protein localization
GO:0034635 : glutathione transport
GO:0030033 : microvillus assembly
GO:0030336 : negative regulation of cell migration
GO:0008285 : negative regulation of cell proliferation
GO:0070373 : negative regulation of ERK1 and ERK2 cascade
GO:0014067 : negative regulation of phosphatidylinositol 3-kinase cascade
GO:0010642 : negative regulation of platelet-derived growth factor receptor signaling pathway
GO:0051898 : negative regulation of protein kinase B signaling cascade
GO:0010766 : negative regulation of sodium ion transport
GO:0032416 : negative regulation of sodium
GO:0060158 : phospholipase C-activating dopamine receptor signaling pathway
GO:0006461 : protein complex assembly
GO:0044062 : regulation of excretion
GO:0045859 : regulation of protein kinase activity
GO:0032415 : regulation of sodium
GO:0097291 : renal phosphate ion absorption
GO:0003096 : renal sodium ion transport
GO:0016055 : Wnt receptor signaling pathway

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease:

OMIM:604990 : Nephrolithiasis/osteoporosis, hypophosphatemic, 2 (OMIM:612287 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC4	NHERF1	Inference						Prediction	10980202
TRPC4	NHERF1	Yeast two-hybrid	Human	686-977	Human	Brain	cDNA library		18048348
TRPC5	NHERF1	Inference						Prediction	10980202

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC4	NHERF1	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	1-102	E. coli	12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	144-247	E. coli	12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Mouse	Full-length	In vitro translation	Mouse	1-107	E. coli	10980202
TRPC5	NHERF1	Fusion protein-pull down assay	Mouse	Full-length	In vitro translation	Mouse	1-107	E. coli	10980202

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC4	NHERF1	Co-immunofluorescence staining		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		18048348
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	12154080
TRPC4	NHERF1	Co-immunoprecipitation		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	NHERF1	Co-immunoprecipitation	Human fetal brain astrocytes						15540229
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		10980202
TRPC4	NHERF1	Co-immunoprecipitation	Mouse brain tissue						10980202

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC4	NHERF1	Co-immunoprecipitation	Human	975-977 (in ơ varint)	Human	130-328			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	975-977 (in ơ varint)	Human	1-102			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	975-977 (in ơ varint)	Human	144-247			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Mouse	688-974	Mouse	1-107			10980202
TRPC5	NHERF1	Fusion protein-pull down assay	Mouse	648-975	Mouse	1-107			10980202

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel		Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC4		NHERF1	Cell surface biotinylation		Increase in plasma membrane level		12154080

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

NHERF1 / TRP channels