TRPC2 / interactors
- At a glance
- In detail
Name: Short transient receptor potential channel 2
(TRPC2)
Official Symbol: Trpc2
provided by HGNC
Function:
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. May also be activated by intracellular calcium store depletion.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
| Human | ||||||||||
Mouse
|
22064 |
Q9R244 |
204328 |
Q9R244 |
487 |
mmu:22064 |
||||
|
Rat
|
64573 |
Q9R283 |
249153 |
Q9R283 |
rno:64573 |
|||||
PPI pairs:
Biological Process:
- GO:0007340
: acrosome reaction - GO:0007204 : elevation of cytosolic calcium ion concentration
- GO:0008050 : female courtship behavior
- GO:0002121 : inter-male aggressive behavior
- GO:0048047 : mating behavior, sex discrimination
- GO:0019236 : response to pheromone
- GO:0000012 : single strand break repair
- GO:0002124 : territorial aggressive behavior
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC2 |
|
Calmodulin | Inference | Prediction | 11290752 | |||||
| TRPC2 |
|
Caspase-4/Caspase-11 | Speculation | Prediction | 24630989 | |||||
| TRPC2 |
|
Enkurin | Yeast two-hybrid | Mouse | 34-526 | Mouse | Testis | cDNA library | 15385169 | |
| TRPC2 |
|
Epo-R | Inference | Prediction | 12167663 | |||||
| TRPC2 |
|
Epo-R | Inference | Prediction | 15329338 | |||||
| TRPC2 |
|
Homer-1 | Inference | Prediction | 14505576 | |||||
| TRPC2 |
|
IP3R2 | Inference | Prediction | 15329338 | |||||
| TRPC2 |
|
IP3R3 | Inference | Prediction | 11290752 | |||||
| TRPC2 |
|
Junctate | Inference | Prediction | 16153633 | |||||
| TRPC2 |
|
PLCƣ1 | Inference | Prediction | 15329338 | |||||
| TRPC2 |
|
RTP1 | Inference | Prediction | 20492691 | |||||
| TRPC2 |
|
SRC | Inference | Prediction | 16407161 | |||||
| TRPC2 |
|
STIM1 | Inference | Prediction | 16906149 | |||||
| TRPC2 |
|
TRPC6 | Inference | Prediction | 14699131 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Not specified | Full-length | In vitro translation | 16153633 |
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 1-619 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
| TRPC2 |
|
Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | E. coli | Mouse | Full-length | HEK293 lysates | 16153633 |
| TRPC2 |
|
SRC | Fusion protein-pull down assay | Mouse | 1-616 | In vitro translation | Chicken | Full-length | E. coli | 16407161 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC2 |
|
Caspase-4/Caspase-11 | Co-immunoprecipitation | HEK293T | Not specified | Full-length | Not specified | Full-length | 24630989 | |
| TRPC2 |
|
Epo-R | Co-immunofluorescence staining | CHO | Mouse | Full-length | Mouse | Full-length | 12167663 | |
| TRPC2 |
|
Epo-R | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Mouse | Full-length | 15329338 | |
| TRPC2 |
|
Epo-R | Co-immunoprecipitation | Mouse erythroblasts | 15329338 | |||||
| TRPC2 |
|
Epo-R | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Mouse | Full-length | 22088247 | |
| TRPC2 |
|
Homer-1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
| TRPC2 |
|
Homer-1 | Co-immunoprecipitation | Rat vomeronasal organ lysates | 20492691 | |||||
| TRPC2 |
|
IP3R2 | Co-immunofluorescence staining | Mouse splenic erythroblast | 15329338 | |||||
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338 | |
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247 | |
| TRPC2 |
|
IP3R3 | Co-immunofluorescence staining | Rat vomeronasal organ | 12472899 | |||||
| TRPC2 |
|
IP3R3 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
| TRPC2 |
|
IP3R3 | Co-immunoprecipitation | Rat vomeronasal organ | 12472899 | |||||
| TRPC2 |
|
PLCƣ1 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 15329338 | |
| TRPC2 |
|
PLCƣ1 | Co-immunoprecipitation | Mouse erythroblasts | 15329338 | |||||
| TRPC2 |
|
PLCƣ1 | Co-immunoprecipitation | CHO-S | Mouse | Full-length | Rat | Full-length | 22088247 | |
| TRPC2 |
|
RTP1 | Co-immunofluorescence staining | Rat vomeronasal organ section | 20492691 | |||||
| TRPC2 |
|
RTP1 | Co-immunoprecipitation | Rat vomeronasal organ lysates | 20492691 | |||||
| TRPC2 |
|
STIM1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Human | Full-length | 16906149 | |
| TRPC2 |
|
TRPC6 | Co-immunoprecipitation | CHO | Mouse | Full-length | Mouse | Full-length | 14699131 | |
| TRPC2 |
|
TRPC6 | Co-immunoprecipitation | Mouse brain erythroblast | 14699131 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC2 |
|
Calmodulin | Fluorescence probe labeling | Mouse | 905-934 | Not specified | Not determined | 10 nM | 11290752 | |
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 104-195 | Not specified | Not determined | 12601176 | ||
| TRPC2 |
|
Calmodulin | Fusion protein-pull down assay | Mouse | 901-936 | Not specified | Not determined | 11290752 | ||
| TRPC2 |
|
IP3R2 | Co-immunoprecipitation | Mouse | 1001-1036 | Rat | Not determined | 15329338 | ||
| TRPC2 |
|
IP3R3 | Fusion protein-pull down assay | Mouse | 901-936 | Human | 669-698 | 11290752 | ||
| TRPC2 |
|
Junctate | Fusion protein-pull down assay | Mouse | 925-1172 | Mouse | 1-25 (in isoform Junctin) | 16153633 | ||
| TRPC2 |
|
STIM1 | Co-immunoprecipitation | Mouse | Not determined | Human | 251-535 | 16906149 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPC2 |
|
Epo-R | Calcium measurement | Activation | 12167663 | |||||||
| TRPC2 |
|
RTP1 | Cell surface biotinylation | Increase in plasma membrane level | 20492691 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)