TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPC4 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (38)
Biological Process (4)
Disease (0)

Name: Short transient receptor potential channel 4 (TRPC4)

Official Symbol: TRPC4 provided by HGNC

Function:

Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

80K-H	Calmodulin	Cav1.2	Caveolin-1	D2R	FKBP5	FKBP52/FKBP4	FYN
GJA1	Gơi2	Homer-3	IP3R1	IP3R2	IP3R3	LYN	MxA
NHERF1	NHERF2	PLCƣ1	Protein 4.1	RNF24	SESTD1	SHANK	STIM1
Src	TARBP2	TRPC1	TRPC3	TRPC5	TRPC6	TRPP1	VASP
VE-cadherin	ZO-1	ơ1-syntrophin	ơII-spectrin	Ƣ-catenin	ƢV-spectrin

Biological Process:

GO:0007411 : axon guidance
GO:0070509 : calcium ion import
GO:0014051 : gamma-aminobutyric acid secretion
GO:0051924 : regulation of calcium ion transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease: No information in OMIM

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC4	80K-H	Speculation						Prediction	19801576
TRPC4	Calmodulin	Inference						Prediction	11290752
TRPC4	Cav1.2	Inference						Prediction	21672930
TRPC4	Caveolin-1	Inference						Prediction	17416589
TRPC4	D2R	Inference						Prediction	18261457
TRPC4	FKBP5	Inference						Prediction	23375350
TRPC4	FKBP52/FKBP4	Inference						Prediction	15199065
TRPC4	FYN	Inference						Prediction	16144838
TRPC4	GJA1	Inference						Prediction	15540229
TRPC4	Gơi2	Inference						Prediction	22457348
TRPC4	Ƣ-catenin	Inference						Prediction	19996314
TRPC4	ƢV-spectrin	Yeast two-hybrid	Human	686-977	Human	Brain	cDNA library		18048348
TRPC4	Homer-3	Inference						Prediction	14505576
TRPC4	IP3R1	Inference						Prediction	11163362
TRPC4	IP3R2	Inference						Prediction	11163362
TRPC4	IP3R3	Inference						Prediction	11290752
TRPC4	LYN	Inference						Prediction	16144838
TRPC4	MxA	Inference						Prediction	15757897
TRPC4	NHERF1	Inference						Prediction	10980202
TRPC4	NHERF1	Yeast two-hybrid	Human	686-977	Human	Brain	cDNA library		18048348
TRPC4	NHERF2	Inference						Prediction	15590898
TRPC4	ơ1-syntrophin	Inference						Prediction	19812031
TRPC4	ơII-spectrin	Yeast two-hybrid	Human	686-977	Human	Brain	cDNA library		18048348
TRPC4	PLCƣ1	Inference						Prediction	12437926
TRPC4	PLCƢ1	Inference						Prediction	10980202
TRPC4	Protein 4.1	Inference						Prediction	16254212
TRPC4	RNF24	Inference						Prediction	17850865
TRPC4	SESTD1	Yeast two-hybrid	Mouse	615-974	Human	Aortic	cDNA library		20164195
TRPC4	SHANK	Inference						Prediction	25049082
TRPC4	SRC	Inference						Prediction	16144838
TRPC4	STIM1	Inference						Prediction	16906149
TRPC4	TARBP2	Yeast two-hybrid	Mouse	Not specified	Mouse	Brain	cDNA library		24563462
TRPC4	TRPC1	Inference						Prediction	11301024
TRPC4	TRPC3	Inference						Prediction	16537542
TRPC4	TRPC5	Inference						Prediction	12032305
TRPC4	TRPC6	Inference						Prediction	12857742
TRPC4	TRPP1	Inference						Prediction	18256307
TRPC4	VASP	Inference						Prediction	17913834
TRPC4	VE-cadherin	Inference						Prediction	19996314
TRPC4	ZO-1	Inference						Prediction	15540229

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC4	Calmodulin	Fusion protein-pull down assay	Not used		Bovine adrenal cortex lysates	Xenopus	Full-length	In vitro translation	11311128
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	1-320	In vitro translation	Not specified	Full-length	E. coli	12601176
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	688-974	In vitro translation	Not specified	Not specified	Not specified	11290752
TRPC4	Caveolin-1	Fusion protein-pull down assay	Not used		Mouse lung endothelium cell lysates	Not specified	Full-length	E. coli	17416589
TRPC4	D2R	Fusion protein-pull down assay	Human	621-893	E. coli	Human	131-151	E. coli	18261457
TRPC4	FKBP52/FKBP4	Co-immunoprecipitation	Bovine	Full-length	Sf9 lysates	Human	Full-length	Sf9 lysates	15199065
TRPC4	Gơi2	Fusion protein-pull down assay	Mouse	Full	E. coli	Human	Full	E. coli	22457348
TRPC4	ƢV-spectrin	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	3232-3674	E. coli	18048348
TRPC4	IP3R1	Yeast two-hybrid	Human	615-977	Yeast	Rat	2520-2670	Yeast	11163362
TRPC4	IP3R2	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Rat	2544-2701	E. coli	11163362
TRPC4	IP3R2	Yeast two-hybrid	Human	615-977	Yeast	Rat	2544-2701	Yeast	11163362
TRPC4	IP3R3	Fusion protein-pull down assay	Mouse	688-974	In vitro translation	Human	669-698	E. coli	11290752
TRPC4	IP3R3	Yeast two-hybrid	Human	615-977	Yeast	Rat	2556-2713	Yeast	11163362
TRPC4	MxA	Fusion protein-pull down assay	Human	3-183	In vitro translation	Human	572-662	E. coli	15757897
TRPC4	NHERF1	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	1-102	E. coli	12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	144-247	E. coli	12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Mouse	Full-length	In vitro translation	Mouse	1-107	E. coli	10980202
TRPC4	ơ1-syntrophin	Fusion protein-pull down assay	Not used		C57BL/10 mouse muscle cell lysates	Mouse	75-170	E. coli	19812031
TRPC4	ơII-spectrin	Fusion protein-pull down assay	Human	Full-length	HEK293 lysates	Human	1998-2472	E. coli	18048348
TRPC4	PLCƣ1	Yeast two-hybrid	Human	1-330	Yeast	Rat	750-1290	Yeast	12437926
TRPC4	RNF24	Fusion protein-pull down assay	Not specified	3-183	In vitro translation	Human	98-148	E. coli	17850865
TRPC4	SRC	Fusion protein-pull down assay	Mouse	626-974	In vitro translation	Chicken	Full-length	E. coli	16407161
TRPC4	TARBP2	Fusion protein-pull down assay	Mouse	625-974	E. coli	Mouse	Full-length	HEK293 lysates	24563462

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC4	80K-H	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Human	Full-length	19801576
TRPC4	Cav1.2	Co-immunoprecipitation	Chick embryonic heart lysates						21672930
TRPC4	Caveolin-1	Co-immunofluorescence staining	Mouse lung endothelium cell						17416589
TRPC4	Caveolin-1	Co-immunoprecipitation	Mouse lung endothelium cell						17416589
TRPC4	FKBP5	Co-immunoprecipitation	Rat pumonary aortic endothelial cell						23375350
TRPC4	FKBP52/FKBP4	Co-immunoprecipitation	Rat cerebral cortex						15199065
TRPC4	FKBP52/FKBP4	Co-immunoprecipitation	Rat pumonary aortic endothelial cell						23375350
TRPC4	FYN	Co-immunoprecipitation	Rat brain microsome						18048348
TRPC4	FYN	Co-immunoprecipitation	COS-7						16144838
TRPC4	FYN	Co-immunoprecipitation		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	GJA1	Co-immunofluorescence staining	Human fetal brain astrocytes						15540229
TRPC4	GJA1	Co-immunoprecipitation	Human fetal brain astrocytes						15540229
TRPC4	Gơi2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	24011658
TRPC4	Gơi2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	22457348
TRPC4	Gơi2	Co-immunoprecipitation	Rat brain extract						22457348
TRPC4	Ƣ-catenin	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Not specified	Full-length	19996314
TRPC4	Ƣ-catenin	Co-immunoprecipitation		Human microvascular endpthelial cell-1	Mouse	Full-length	Not specified	Full-length	19996314
TRPC4	Ƣ-catenin	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Not specified	Full-length	19996314
TRPC4	Homer-3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		14505576
TRPC4	IP3R3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		14505576
TRPC4	LYN	Co-immunoprecipitation	COS-7						16144838
TRPC4	LYN	Co-immunoprecipitation		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	MxA	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	15757897
TRPC4	NHERF1	Co-immunofluorescence staining		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		18048348
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	12154080
TRPC4	NHERF1	Co-immunoprecipitation		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	NHERF1	Co-immunoprecipitation	Human fetal brain astrocytes						15540229
TRPC4	NHERF1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		10980202
TRPC4	NHERF1	Co-immunoprecipitation	Mouse brain tissue						10980202
TRPC4	NHERF2	Co-immunofluorescence staining	Rat kidney tissue						15590898
TRPC4	NHERF2	Co-immunoprecipitation	Rat kidney tissue						15590898
TRPC4	ơ1-syntrophin	Co-immunoprecipitation	C57BL/10 mouse muscle cell						19812031
TRPC4	ơII-spectrin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		18048348
TRPC4	PLCƢ1	Co-immunofluorescence staining		Neonatal rat ventricular myocytes	Not used		Mouse	Full-length	25049082
TRPC4	PLCƢ1	Co-immunoprecipitation		Neonatal rat ventricular myocytes	Human	Full-length	Mouse	Full-length	25049082
TRPC4	PLCƢ1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		10980202
TRPC4	PLCƢ1	Co-immunoprecipitation	Mouse brain tissue						10980202
TRPC4	Protein 4.1	Co-immunoprecipitation	Rat pumonary aortic endothelial cell						16254212
TRPC4	Protein 4.1	Co-immunoprecipitation	Rat pumonary aortic endothelial cell						22534489
TRPC4	SESTD1	Co-immunoprecipitation		HEK293-HM1 stable cell line	Mouse	Full-length	Human	Full-length	20164195
TRPC4	SHANK	Co-immunoprecipitation		Neonatal rat ventricular myocytes	Human	Full-length	Not used		25049082
TRPC4	SRC	Co-immunoprecipitation		COS-7	Human	Full-length	Not specifed	Full-length	16144838
TRPC4	STIM1	Co-immunoprecipitation	Human glomerular mesangial cell						19307462
TRPC4	STIM1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	16906149
TRPC4	STIM1	Co-immunoprecipitation		HEK293	Not specified	Full-length	Human	Full-length	24464579
TRPC4	STIM1	Co-immunoprecipitation		Mouse lung endothelial cell	Mouse	Full-length	Human	Full-length	22210847
TRPC4	TARBP2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	24563462
TRPC4	TRPC1	Co-immunofluorescence staining	BAE-1						16818374
TRPC4	TRPC1	Co-immunofluorescence staining	Human glomerular mesangial cell						16418302
TRPC4	TRPC1	Co-immunoprecipitation	Rat brain microsome						11301024
TRPC4	TRPC1	Co-immunoprecipitation	BAE-1						16818374
TRPC4	TRPC1	Co-immunoprecipitation	Human glomerular mesangial cell						19307462
TRPC4	TRPC1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	12032305
TRPC4	TRPC1	Co-immunoprecipitation	C57BL/10 mouse muscle cell						19812031
TRPC4	TRPC1	Co-immunoprecipitation		Sf9	Bovine	Full-length	Human	Full-length	12377790
TRPC4	TRPC1	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC4	TRPC1	Co-immunoprecipitation	Human platelets						16270640
TRPC4	TRPC1	Co-immunoprecipitation	Mouse lung endothelium cell						17416589
TRPC4	TRPC1	Co-immunoprecipitation	Human glomerular mesangial cell						16418302
TRPC4	TRPC1	Co-immunoprecipitation		Mouse lung endothelial cell	Mouse	Full-length	Mouse	Full-length	22210847
TRPC4	TRPC1	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC4	TRPC1	Co-immunoprecipitation	Human platelets						17892531
TRPC4	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	12032305
TRPC4	TRPC1	Fluorescence resonance energy transfer	Rat pumonary aortic endothelial cell						22534489
TRPC4	TRPC3	Co-immunofluorescence staining	Porcine primary aortic endothelial cell						16537542
TRPC4	TRPC3	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC4	TRPC3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	16537542
TRPC4	TRPC3	Co-immunoprecipitation	Porcine primary aortic endothelial cell						16537542
TRPC4	TRPC3	Co-immunoprecipitation	TRPC3 dnTRPC4 DTG mouse heart lysataes						20351294
TRPC4	TRPC5	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	12032305
TRPC4	TRPC5	Co-immunoprecipitation		Sf9	Bovine	Full-length	Mouse	Full-length	12377790
TRPC4	TRPC5	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC4	TRPC5	Co-immunoprecipitation	Human platelets						16270640
TRPC4	TRPC5	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	12032305
TRPC4	TRPC5	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	17624425
TRPC4	TRPC6	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not specifed	Full-length	17486119
TRPC4	TRPC6	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC4	TRPP1	Co-immunoprecipitation		Human glomerular mesangial cell	Bovine	Full-length	Not used		18256307
TRPC4	VASP	Co-immunofluorescence staining	Human glomerular mesangial cell						17913834
TRPC4	VASP	Co-immunoprecipitation	Human glomerular mesangial cell						17913834
TRPC4	VE-cadherin	Co-immunoprecipitation		Human microvascular endpthelial cell-1	Mouse	Full-length	Mouse	Full-length	19996314
TRPC4	VE-cadherin	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	19996314
TRPC4	ZO-1	Co-immunofluorescence staining	Human fetal brain astrocytes						15540229
TRPC4	ZO-1	Co-immunoprecipitation	Human fetal brain astrocytes						15540229

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC4	Calmodulin	Fluorescence probe labeling	Mouse	694-718	Bovine	Not determined		94 nM	11311128
TRPC4	Calmodulin	Fluorescence probe labeling	Mouse	829-853	Bovine	Not determined		138 nM	11311128
TRPC4	Calmodulin	Fluorescence probe labeling	Mouse	708-728	Bovine	Not determined		189 nM	11311128
TRPC4	Calmodulin	Fluorescence probe labeling	Mouse	695-724	Not specified	Not determined		200 nM	11290752
TRPC4	Calmodulin	Fluorescence probe labeling	Mouse	787-812	Not specified	Not determined		600 nM	11290752
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	786-848	Not specified	Not determined			11311128
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	688-759	Not specified	Not determined			11311128
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	695-724	Not specified	Not determined			15104175
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	789-812	Not specified	Not determined			11290752
TRPC4	Calmodulin	Fusion protein-pull down assay	Mouse	695-724	Not specified	Not determined			11290752
TRPC4	Caveolin-1	Fusion protein-pull down assay	Mouse	Not determined	Not specified	61-101			17416589
TRPC4	Caveolin-1	Fusion protein-pull down assay	Mouse	Not determined	Not specified	135-178			17416589
TRPC4	D2R	Yeast two-hybrid	Human	712-731	Human	131-151			18261457
TRPC4	Gơi2	Co-immunoprecipitation	Mouse	700-728	Human	Not determined			22457348
TRPC4	ƢV-spectrin	Fusion protein-pull down assay	Human	686-977	Human	3232-3674			18048348
TRPC4	ƢV-spectrin	Yeast two-hybrid	Human	686-977	Human	3232-3674			18048348
TRPC4	IP3R2	Fusion protein-pull down assay	Human	785-868	Rat	2556-2713			11163362
TRPC4	IP3R3	Fusion protein-pull down assay	Mouse	695-724	Human	669-698			15104175
TRPC4	IP3R3	Fusion protein-pull down assay	Mouse	781-864	Human	669-698			11290752
TRPC4	IP3R3	Fusion protein-pull down assay	Mouse	695-724	Human	669-698			11290752
TRPC4	MxA	Fusion protein-pull down assay	Human	3-183	Human	572-662			15757897
TRPC4	NHERF1	Co-immunoprecipitation	Human	975-977 (in ơ varint)	Human	130-328			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	975-977 (in ơ varint)	Human	1-102			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Human	975-977 (in ơ varint)	Human	144-247			12154080
TRPC4	NHERF1	Fusion protein-pull down assay	Mouse	688-974	Mouse	1-107			10980202
TRPC4	ơ1-syntrophin	Fusion protein-pull down assay	Mouse	Not determined	Mouse	75-170			19812031
TRPC4	ơII-spectrin	Fusion protein-pull down assay	Human	730-758	Human	2202-2313			18048348
TRPC4	ơII-spectrin	Yeast two-hybrid	Human	686-859	Human	1998-2472			18048348
TRPC4	PLCƣ1	Yeast two-hybrid	Human	1-330	Rat	780-920			12437926
TRPC4	SESTD1	Fusion protein-pull down assay	Mouse	700-728	Human	193-406			20164195
TRPC4	STIM1	Co-immunoprecipitation	Mouse	Not determined	Human	251-535			16906149
TRPC4	TARBP2	Co-immunoprecipitation	Mouse	625-974	Mouse	209-234			24563462
TRPC4	TRPC5	Fluorescence resonance energy transfer	Mouse	1-292	Mouse	1-308			17624425
TRPC4	TRPC6	Co-immunoprecipitation	Mouse	1-304	Mouse	715-930			16916799
TRPC4	ZO-1	Fusion protein-pull down assay	Human	890-892	Not specified	First PDZ domain			15540229

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC4	Gơi2	Patch clamp			Activation	22457348
TRPC4	Ƣ-catenin	Calcium measurement			Activation	19996314
TRPC4	NHERF1	Cell surface biotinylation		Increase in plasma membrane level		12154080
TRPC4	ơII-spectrin	Cell surface biotinylation		Increase in plasma membrane level		18048348
TRPC4	Protein 4.1	Calcium measurement			Activation	16254212
TRPC4	Protein 4.1	Patch clamp			Activation	16254212
TRPC4	RNF24	Cell surface biotinylation		Decrease in plasma membrane level		17850865
TRPC4	TRPC1	Patch clamp			New channel creation	11301024
TRPC4	TRPC1	Patch clamp			New channel creation	22144671

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPC4 / interactors