TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPC5 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (59)
Biological Process (1)
Disease (0)

Name: Short transient receptor potential channel 5 (TRPC5)

Official Symbol: TRPC5 provided by HGNC

Function:

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

AP2B1	CAND1	CaBP1	CaMKIIƢ	Calmodulin	Cav1.2	Clathrin-H1	D2R
Drebrin	Dynamin-1	EF2	Enkurin	FKBP52/FKBP4	Gơi3	Homer-1	Homer-3
IP3R3	Junctate	MxA	Myosin-10	Myosin-5a	Myosin-HIIA	NCS-1	NHERF1
NKAơ1	NKAơ3	Neurabin-1	Neurabin-2	OGDH	RNF24	RyR1	SEC31A
SESTD1	SNAP91	SRA-1	STIM1	Src	Stathmin1	Stathmin2	Stathmin3
Stathmin4	TARBP2	TRF1	TRF2	TRPC1	TRPC3	TRPC4	TRPC6
TRPC7	TUBGCP2	TUBGCP3	UBC	ơ-actinin	ơ-actinin-1	ơ-actinin-4	ơ2-actin
ơII-spectrin	ƢII-spectrin	ƢIII-spectrin

Biological Process:

GO:0007411 : axon guidance

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease: No information in OMIM

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC5	AP2B1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	CaBP1	Inference						Prediction	15895247
TRPC5	Calmodulin	Inference						Prediction	11290752
TRPC5	CaMKIIƢ	Inference						Prediction	22135323
TRPC5	CAND1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Cav1.2	Inference						Prediction	21672930
TRPC5	Clathrin-H1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	D2R	Inference						Prediction	18261457
TRPC5	Drebrin	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Dynamin-1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	EF2	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Enkurin	Inference						Prediction	15385169
TRPC5	FKBP52/FKBP4	Inference						Prediction	15199065
TRPC5	Gơi3	Inference						Prediction	22457348
TRPC5	ƢII-spectrin	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	ƢIII-spectrin	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Homer-1	Inference						Prediction	14505576
TRPC5	Homer-3	Inference						Prediction	14505576
TRPC5	IP3R3	Inference						Prediction	11290752
TRPC5	Junctate	Inference						Prediction	16153633
TRPC5	MxA	Inference						Prediction	15757897
TRPC5	Myosin-10	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Myosin-5a	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Myosin-HIIA	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	NCS-1	Inference						Prediction	16469785
TRPC5	Neurabin-1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	Neurabin-2	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	NHERF1	Inference						Prediction	10980202
TRPC5	NKAơ1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	NKAơ3	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	ơ-actinin	Speculation						Prediction	16025302
TRPC5	ơ-actinin-1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	ơ-actinin-4	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	ơ2-actin	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	OGDH	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	ơII-spectrin	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	RNF24	Inference						Prediction	17850865
TRPC5	RyR1	Affinity purification-mass spectrometry	Not used as a bait		Rabbit	Fast-twitch muscle	Triad vesicles		18215135
TRPC5	SEC31A	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	SESTD1	Inference						Prediction	20164195
TRPC5	SNAP91	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	SRA-1	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	SRC	Inference						Prediction	16407161
TRPC5	Stathmin1	Inference						Prediction	12858178
TRPC5	Stathmin2	Inference						Prediction	12858178
TRPC5	Stathmin3	Yeast two-hybrid	Mouse	1-333	Rat	Brain	cDNA library		12858178
TRPC5	Stathmin4	Inference						Prediction	12858178
TRPC5	STIM1	Inference						Prediction	16906149
TRPC5	TARBP2	Inference						Prediction	24563462
TRPC5	TRF1	Affinity purification-mass spectrometry	Not used as a bait		Human	HEK293T	Cell lystaes		20811636
TRPC5	TRF2	Affinity purification-mass spectrometry	Not used as a bait		Human	HEK293T	Cell lystaes		20811636
TRPC5	TRPC1	Inference						Prediction	11301024
TRPC5	TRPC3	Inference						Prediction	12857742
TRPC5	TRPC4	Inference						Prediction	12032305
TRPC5	TRPC6	Inference						Prediction	12857742
TRPC5	TRPC7	Inference						Prediction	18356201
TRPC5	TUBGCP2	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	TUBGCP3	Affinity purification-mass spectrometry	Endogenous protein		Rat	Brain	Cerebral cortex lysates		16025302
TRPC5	UBC	Affinity purification-mass spectrometry	Not used as a bait		Human	U2OS osteosarcoma	Cell lystaes		21139048

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC5	CaBP1	Fusion protein-pull down assay	Mouse	Full-length	E. coli	Human	Full-length	E. coli	15895247
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	1-331	In vitro translation	Not specified	Not specified	Not specified	15987684
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	701-807	In vitro translation	Not specified	Not specified	Not specified	15987684
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	762-975	In vitro translation	Not specified	Not specified	Not specified	15987684
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	1-321	In vitro translation	Not specified	Full-length	E. coli	12601176
TRPC5	D2R	Fusion protein-pull down assay	Human	619-973	E. coli	Human	131-151	E. coli	18261457
TRPC5	Enkurin	Fusion protein-pull down assay	Mouse	Full-length	E. coli	Mouse	Full-length	HEK293 lysates	15385169
TRPC5	Enkurin	Yeast two-hybrid	Mouse	N-terminus	Yeast	Mouse	Full-length	Yeast	15385169
TRPC5	FKBP52/FKBP4	Co-immunoprecipitation	Mouse	Full-length	Sf9 lysates	Human	Full-length	Sf9 lysates	15199065
TRPC5	Gơi3	Fusion protein-pull down assay	Human	Full	E. coli	Human	Full	E. coli	22457348
TRPC5	Junctate	Fusion protein-pull down assay	Not specified	Full-length	HEK293 lysates	Mouse	Full-length	In vitro translation	16153633
TRPC5	MxA	Fusion protein-pull down assay	Human	1-183	In vitro translation	Human	572-662	E. coli	15757897
TRPC5	NCS-1	Fusion protein-pull down assay	Human	619-973	E. coli	Human	Full-length	E. coli	16469785
TRPC5	NCS-1	Yeast two-hybrid	Human	619-973	Yeast	Human	Full-length	Yeast	16469785
TRPC5	NHERF1	Fusion protein-pull down assay	Mouse	Full-length	In vitro translation	Mouse	1-107	E. coli	10980202
TRPC5	RNF24	Fusion protein-pull down assay	Not specified	1-183	In vitro translation	Human	98-148	E. coli	17850865
TRPC5	SRC	Fusion protein-pull down assay	Mouse	630-975	In vitro translation	Chicken	Full-length	E. coli	16407161
TRPC5	SRC	Fusion protein-pull down assay	Mouse	1-321	In vitro translation	Chicken	Full-length	E. coli	16407161
TRPC5	Stathmin3	Fusion protein-pull down assay	Not specified	Full-length	In vitro translation	Not specified	Full-length	In vitro translation	12858178

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC5	CaMKIIƢ	Co-immunoprecipitation	Whole-brain lysates						22135323
TRPC5	CaMKIIƢ	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Rat	Full-length	22135323
TRPC5	CaMKIIƢ	Co-immunoprecipitation	cortical neurons						22135323
TRPC5	Cav1.2	Co-immunoprecipitation	Chick embryonic heart lysates						21672930
TRPC5	Clathrin-H1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC5	Drebrin	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC5	Dynamin-1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC5	FKBP52/FKBP4	Co-immunoprecipitation	Rat cerebral cortex						15199065
TRPC5	Gơi3	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	22457348
TRPC5	Gơi3	Co-immunoprecipitation	Rat brain extract						22457348
TRPC5	Homer-1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		14505576
TRPC5	Homer-3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		14505576
TRPC5	IP3R3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		14505576
TRPC5	NCS-1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	16469785
TRPC5	NCS-1	Co-immunoprecipitation	Rat brain membrane lysates						16469785
TRPC5	NKAơ1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC5	ơ-actinin	Co-immunofluorescence staining	Rat ventricular myocytes						24146259
TRPC5	ơ-actinin	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC5	SESTD1	Co-immunoprecipitation		HEK293-HM1 stable cell line	Mouse	Full-length	Human	Full-length	20164195
TRPC5	Stathmin1	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not specified	Full-length	12858178
TRPC5	Stathmin2	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not specified	Full-length	12858178
TRPC5	Stathmin3	Co-immunoprecipitation		HEK293T	Not specified	Full-length	Not specified	Full-length	12858178
TRPC5	Stathmin4	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not specified	Full-length	12858178
TRPC5	STIM1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	16906149
TRPC5	STIM1	Co-immunoprecipitation		HEK293	Not specified	Full-length	Human	Full-length	24464579
TRPC5	STIM1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		17486119
TRPC5	STIM1	Co-immunoprecipitation		HEK293T	Rat	Full-length	Rat	Full-length	22201561
TRPC5	TARBP2	Co-immunoprecipitation		HEK293	Not specified	Not specified	Mouse	Full-length	24563462
TRPC5	TRPC1	Co-immunoprecipitation	Rat brain microsome						11301024
TRPC5	TRPC1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	11301024
TRPC5	TRPC1	Co-immunoprecipitation		Sf9	Mouse	Full-length	Human	Full-length	12377790
TRPC5	TRPC1	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC5	TRPC1	Co-immunoprecipitation	Rabbit coronary artery						18356201
TRPC5	TRPC1	Co-immunoprecipitation	Rabbit mesenteric artery						18356201
TRPC5	TRPC1	Co-immunoprecipitation	Rabbit portal vein						18356201
TRPC5	TRPC1	Co-immunoprecipitation	Human platelets						16270640
TRPC5	TRPC1	Co-immunoprecipitation	Mouse vascular smooth muscle cell						21968068
TRPC5	TRPC1	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC5	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	12032305
TRPC5	TRPC3	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC5	TRPC4	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	12032305
TRPC5	TRPC4	Co-immunoprecipitation		Sf9	Mouse	Full-length	Bovine	Full-length	12377790
TRPC5	TRPC4	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC5	TRPC4	Co-immunoprecipitation	Human platelets						16270640
TRPC5	TRPC4	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	12032305
TRPC5	TRPC4	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	17624425
TRPC5	TRPC6	Co-immunoprecipitation	Rabbit coronary artery						18356201
TRPC5	TRPC6	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC5	TRPC7	Co-immunoprecipitation	Rabbit portal vein						18356201

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC5	CaBP1	Fusion protein-pull down assay	Mouse	1-331	Human	Not determined			15895247
TRPC5	CaBP1	Fusion protein-pull down assay	Mouse	721-817	Human	Not determined			15895247
TRPC5	CaBP1	Fusion protein-pull down assay	Mouse	791-866	Human	Not determined			15895247
TRPC5	Calmodulin	Fluorescence probe labeling	Mouse	702-731	Not specified	Not determined		220 nM	11290752
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	701-733	Bovine	Not determined			15987684
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	828-854	Bovine	Not determined			15987684
TRPC5	Calmodulin	Fusion protein-pull down assay	Mouse	701-733	Not specified	Not determined			11290752
TRPC5	CaMKIIƢ	Co-immunoprecipitation	Mouse	N-terminus	Rat	Not determined			22135323
TRPC5	CaMKIIƢ	Co-immunoprecipitation	Mouse	Not determined	Rat	C-terminus			22135323
TRPC5	D2R	Yeast two-hybrid	Human	719-738	Human	131-151			18261457
TRPC5	Gơi3	Co-immunoprecipitation	Human	Not determined	Human	Not determined			22457348
TRPC5	IP3R3	Fusion protein-pull down assay	Mouse	701-733	Human	669-698			11290752
TRPC5	MxA	Fusion protein-pull down assay	Human	1-183	Human	572-662			15757897
TRPC5	NCS-1	Fusion protein-pull down assay	Human	619-803	Human	Not determined			16469785
TRPC5	NCS-1	Fusion protein-pull down assay	Human	803-973	Human	Not determined			16469785
TRPC5	NCS-1	Yeast two-hybrid	Human	619-803	Human	Not determined			16469785
TRPC5	NCS-1	Yeast two-hybrid	Human	803-973	Human	Not determined			16469785
TRPC5	NHERF1	Fusion protein-pull down assay	Mouse	648-975	Mouse	1-107			10980202
TRPC5	SESTD1	Fusion protein-pull down assay	Mouse	707-735	Human	193-406			20164195
TRPC5	STIM1	Co-immunoprecipitation	Rat	Not determined	Rat	1798-1887			22201561
TRPC5	TRPC4	Fluorescence resonance energy transfer	Mouse	1-308	Mouse	1-292			17624425

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC5	CaBP1	Patch clamp			Inhibition	15895247
TRPC5	Gơi3	Patch clamp			Activation	22457348
TRPC5	NCS-1	Calcium measurement			Activation	16469785
TRPC5	NCS-1	Patch clamp			Activation	16469785
TRPC5	SESTD1	Calcium measurement			Activation	20164195
TRPC5	TRPC1	Patch clamp			New channel creation	11301024
TRPC5	TRPC1	Patch clamp			New channel creation	12857742

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPC5 / interactors