TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPC6 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (46)
Biological Process (6)
Disease (1)

Name: Short transient receptor potential channel 6 (TRPC6)

Official Symbol: TRPC6 provided by HGNC

Function:

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

Bkca	Calcineurin	Calmodulin	Cav1.2	Clathrin-H1	Drebrin	Dynamin-1	Epo-R
FKBP12	FYN	GC-A	Gơq/11	IP3R2	IP3R3	M1 mAChR	MxA
NKAơ1	Nephrin	Orai1	Orai2	Orai3	PDE3A	PKA	PKCơ
PKG1	PLCƣ1	Podocin	RNF24	Rab9	S100A10	SERCA2	SERCA3
SNAPIN	SNF8	STIM1	Src	Syntaxin3	TRPA1	TRPC1	TRPC2
TRPC3	TRPC4	TRPC5	TRPC7	UBC	ơ-actinin

Biological Process:

GO:0007411 : axon guidance
GO:0006812 : cation transport
GO:0007204 : elevation of cytosolic calcium ion concentration
GO:0030168 : platelet activation
GO:0051928 : positive regulation of calcium ion transport
GO:0032414 : positive regulation of ion transmembrane transporter activity

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease:

OMIM:603652 : Glomerulosclerosis, focal segmental, 2 (OMIM:603965 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPC6	BKca	Inference						Prediction	19052171
TRPC6	Calcineurin	Inference						Prediction	15994335
TRPC6	Calmodulin	Inference						Prediction	11290752
TRPC6	Cav1.2	Inference						Prediction	21672930
TRPC6	Clathrin-H1	Speculation						Prediction	16025302
TRPC6	Drebrin	Speculation						Prediction	16025302
TRPC6	Dynamin-1	Speculation						Prediction	16025302
TRPC6	Epo-R	Inference						Prediction	19074769
TRPC6	FKBP12	Inference						Prediction	15994335
TRPC6	FYN	Inference						Prediction	14761972
TRPC6	GC-A	Inference						Prediction	22027011
TRPC6	Gơq/11	Inference						Prediction	15623527
TRPC6	IP3R2	Inference						Prediction	18191041
TRPC6	IP3R3	Inference						Prediction	10611319
TRPC6	M1 mAChR	Inference						Prediction	15994335
TRPC6	MxA	Yeast two-hybrid	Human	3-259	Human	Fetal brain	cDNA library		15757897
TRPC6	Nephrin	Inference						Prediction	15924139
TRPC6	NKAơ1	Speculation						Prediction	16025302
TRPC6	ơ-actinin	Speculation						Prediction	16025302
TRPC6	Orai1	Inference						Prediction	17360584
TRPC6	Orai2	Speculation						Prediction	22640869
TRPC6	Orai3	Speculation						Prediction	22640869
TRPC6	PDE3A	Inference						Prediction	21799177
TRPC6	PKA	Inference						Prediction	21799177
TRPC6	PKCơ	Inference						Prediction	15994335
TRPC6	PKG1	Inference						Prediction	18617565
TRPC6	PLCƣ1	Inference						Prediction	19074769
TRPC6	Podocin	Inference						Prediction	15924139
TRPC6	Rab9	Inference						Prediction	20346379
TRPC6	RNF24	Yeast two-hybrid	Not specified	3-259	Human	Fetal brain	cDNA library		17850865
TRPC6	S100A10	Inference						Prediction	23671622
TRPC6	SERCA2	Inference						Prediction	18191041
TRPC6	SERCA3	Inference						Prediction	18191041
TRPC6	SNAPIN	Inference						Prediction	17684020
TRPC6	SNF8	Yeast two-hybrid	Human	1-406	Human	Kidney	cDNA library		23171048
TRPC6	SRC	Inference						Prediction	16407161
TRPC6	Src	Speculation						Prediction	14761972
TRPC6	STIM1	Inference						Prediction	19260825
TRPC6	Syntaxin3	Inference						Prediction	15623527
TRPC6	TRPA1	Inference						Prediction	23994313
TRPC6	TRPC1	Inference						Prediction	12857742
TRPC6	TRPC2	Inference						Prediction	14699131
TRPC6	TRPC3	Inference						Prediction	12032305
TRPC6	TRPC4	Inference						Prediction	12857742
TRPC6	TRPC5	Inference						Prediction	12857742
TRPC6	TRPC7	Inference						Prediction	12032305
TRPC6	UBC	Inference						Prediction	17110928

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPC6	BKca	Fusion protein-pull down assay	Not used		Mouse podocyte lysates	Chicken	Full-length	E. coli	19052171
TRPC6	Calmodulin	Fusion protein-pull down assay	Mouse	828-883	E. coli	Not specified	Full-length	In vitro translation	12379180
TRPC6	Calmodulin	Fusion protein-pull down assay	Mouse	1-396	In vitro translation	Not specified	Full-length	E. coli	12601176
TRPC6	FKBP12	Co-immunoprecipitation	Mouse	Full-length	Sf9 lysates	Human	Full-length	Sf9 lysates	15199065
TRPC6	FYN	Fusion protein-pull down assay	Mouse	Full-length	HEK293 lysates	Human	149-257	E. coli	14761972
TRPC6	MxA	Fusion protein-pull down assay	Human	95-243	In vitro translation	Human	572-662	E. coli	15757897
TRPC6	Orai1	Fusion protein-pull down assay	Mouse	1-396	In vitro translation	Human	Full-length	E. coli	17360584
TRPC6	Orai1	Fusion protein-pull down assay	Mouse	732-930	In vitro translation	Human	Full-length	E. coli	17360584
TRPC6	RNF24	Fusion protein-pull down assay	Not specified	3-259	In vitro translation	Human	98-148	E. coli	17850865
TRPC6	RNF24	Yeast two-hybrid	Not specified	3-259	Yeast	Human	98-148	Yeast	17850865
TRPC6	S100A10	Fluorescence probe labeling	Rat	801-878	E. coli	Human	Full-length	E. coli	23671622
TRPC6	SRC	Fusion protein-pull down assay	Mouse	1-396	In vitro translation	Chicken	Full-length	E. coli	16407161

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPC6	BKca	Co-immunofluorescence staining		HEK293T	Not specified	Full-length	Not specified	Full-length	19052171
TRPC6	BKca	Co-immunoprecipitation	Mouse podocyte lysates						19052171
TRPC6	BKca	Co-immunoprecipitation		HEK293T	Not specified	Full-length	Not specified	Full-length	19052171
TRPC6	Calcineurin	Co-immunoprecipitation	PC12D						15994335
TRPC6	Calmodulin	Co-immunoprecipitation	PC12D						15994335
TRPC6	Cav1.2	Co-immunoprecipitation	Chick embryonic heart lysates						21672930
TRPC6	Clathrin-H1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC6	Drebrin	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC6	Dynamin-1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC6	Epo-R	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	19074769
TRPC6	FKBP12	Co-immunoprecipitation	PC12D						15994335
TRPC6	FKBP12	Co-immunoprecipitation	Rat cerebral cortex						15199065
TRPC6	FYN	Co-immunofluorescence staining		CHO-K1	Mouse	Full-length	Human	Full-length	14761972
TRPC6	FYN	Co-immunoprecipitation	Rat brain microsome						14761972
TRPC6	FYN	Co-immunoprecipitation		COS-7	Mouse	Full-length	Human	Full-length	14761972
TRPC6	GC-A	Co-immunoprecipitation		HEK293	Not specified	Full-length	Rat	Full-length	22027011
TRPC6	GC-A	Co-immunoprecipitation		Mouse cardiac myocyte lysates	Not used		Rat	Full-length	22027011
TRPC6	Gơq/11	Co-immunoprecipitation	MDCK						15623527
TRPC6	IP3R2	Co-immunoprecipitation	Human platelets						18191041
TRPC6	IP3R3	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		14505576
TRPC6	IP3R3	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Human	Full-length	10611319
TRPC6	M1 mAChR	Co-immunoprecipitation	PC12D						15994335
TRPC6	MxA	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	15757897
TRPC6	Nephrin	Co-immunofluorescence staining		Mouse podocytes section	Mouse	Full-length	Not used		15924139
TRPC6	Nephrin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	15924139
TRPC6	Nephrin	Co-immunoprecipitation	Mouse podocyte lysates						15924139
TRPC6	Nephrin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	22155451
TRPC6	Nephrin	Co-immunoprecipitation	Mouse podocyte lysates						22155451
TRPC6	NKAơ1	Co-immunofluorescence staining		HEK293	Human	Full-length	Not used		16025302
TRPC6	NKAơ1	Co-immunoprecipitation	Rat kidney lysates						16025302
TRPC6	NKAơ1	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC6	ơ-actinin	Co-immunofluorescence staining	Rat ventricular myocytes						24146259
TRPC6	ơ-actinin	Co-immunoprecipitation	Rat brain lysates						16025302
TRPC6	Orai1	Co-immunoprecipitation	Human platelets						19260825
TRPC6	Orai1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	17360584
TRPC6	Orai2	Co-immunoprecipitation	Human platelets						22640869
TRPC6	Orai3	Co-immunoprecipitation	Human platelets						22640869
TRPC6	PDE3A	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		21799177
TRPC6	PDE3A	Co-immunoprecipitation	Rat aortic smooth muscle cell						21799177
TRPC6	PKA	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		21799177
TRPC6	PKA	Co-immunoprecipitation		HEK293T	Human	Full-length	Not specified	Not specified	23645677
TRPC6	PKCơ	Co-immunoprecipitation	PC12D						15994335
TRPC6	PLCƣ1	Co-immunoprecipitation		HEK293T	Human	Full-length	Rat	Full-length	19074769
TRPC6	Podocin	Co-immunofluorescence staining		Mouse podocytes section	Mouse	Full-length	Not used		15924139
TRPC6	Podocin	Co-immunoprecipitation		HEK293T	Human	Full-length	Mouse	Full-length	24596097
TRPC6	Podocin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	15924139
TRPC6	Podocin	Co-immunoprecipitation	Mouse podocyte lysates						15924139
TRPC6	Rab9	Co-immunofluorescence staining		HeLa	Mouse	Full-length	Human	Full-length	20346379
TRPC6	Rab9	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	20346379
TRPC6	RNF24	Co-immunofluorescence staining		HEK293	Not specified	Full-length	Human	Full-length	17850865
TRPC6	SERCA2	Co-immunoprecipitation	Human platelets						18191041
TRPC6	SERCA3	Co-immunoprecipitation	Human platelets						18191041
TRPC6	SNAPIN	Co-immunoprecipitation	Rat brain cortex						17684020
TRPC6	SNF8	Co-immunofluorescence staining		M1R-HEK293T	Human	Full-length	Human	Full-length	23171048
TRPC6	SNF8	Co-immunoprecipitation		M1R-HEK293T	Human	Full-length	Human	Full-length	23171048
TRPC6	Src	Co-immunoprecipitation	Rat brain microsome						14761972
TRPC6	STIM1	Co-immunoprecipitation	Human platelets						19260825
TRPC6	STIM1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	23068106
TRPC6	STIM1	Co-immunoprecipitation			Not specified	Full-length (mutant)	Human	Full-length	24464579
TRPC6	Syntaxin3	Co-immunoprecipitation	MDCK						15623527
TRPC6	TRPA1	Co-immunoprecipitation	MEG-01						23994313
TRPC6	TRPC1	Co-immunofluorescence staining	Human glomerular mesangial cell						16418302
TRPC6	TRPC1	Co-immunoprecipitation	Human platelets						18191041
TRPC6	TRPC1	Co-immunoprecipitation	Human glomerular mesangial cell						16418302
TRPC6	TRPC1	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC6	TRPC2	Co-immunoprecipitation		CHO	Mouse	Full-length	Mouse	Full-length	14699131
TRPC6	TRPC2	Co-immunoprecipitation	Mouse brain erythroblast						14699131
TRPC6	TRPC3	Co-immunofluorescence staining	MDA-MB-231						19689790
TRPC6	TRPC3	Co-immunoprecipitation	dnTRPC6 Neonatal rat ventricular myocytes						20351294
TRPC6	TRPC3	Co-immunoprecipitation	Human platelets						19260825
TRPC6	TRPC3	Co-immunoprecipitation	MDA-MB-231						19689790
TRPC6	TRPC3	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	12032305
TRPC6	TRPC3	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC6	TRPC3	Co-immunoprecipitation		Sf9	Mouse	Full-length	Human	Full-length	12377790
TRPC6	TRPC3	Co-immunoprecipitation	Human platelets						16270640
TRPC6	TRPC3	Co-immunoprecipitation		MDCK	Not used		Not specified	Full-length	15623527
TRPC6	TRPC3	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	19074769
TRPC6	TRPC3	Co-immunoprecipitation	TF-1						19074769
TRPC6	TRPC3	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Human	Full-length	12032305
TRPC6	TRPC4	Co-immunoprecipitation		HEK293	Not specifed	Full-length	Mouse	Full-length	17486119
TRPC6	TRPC4	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC6	TRPC5	Co-immunoprecipitation	Rabbit coronary artery						18356201
TRPC6	TRPC5	Co-immunoprecipitation	Rat brain microsome						12857742
TRPC6	TRPC7	Co-immunoprecipitation		Sf9	Mouse	Full-length	Mouse	Full-length	12377790
TRPC6	TRPC7	Co-immunoprecipitation	Rat brain synaptome						12377790
TRPC6	TRPC7	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Mouse	Full-length	12032305

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPC6	Calmodulin	Fluorescence probe labeling						320 nM	19932145
TRPC6	Calmodulin	Fluorescence probe labeling	Mouse	843-872	Not specified	Not determined		290 nM	11290752
TRPC6	Calmodulin	Fusion protein-pull down assay	Mouse	838-872	Not specified	Not determined			11290752
TRPC6	FYN	Fusion protein-pull down assay	Mouse	1-388	Human	149-257			14761972
TRPC6	IP3R3	Fusion protein-pull down assay	Mouse	838-872	Human	669-698			11290752
TRPC6	MxA	Fusion protein-pull down assay	Human	95-243	Human	572-662			15757897
TRPC6	MxA	Yeast two-hybrid	Human	3-259	Human	542-662			15757897
TRPC6	Rab9	Fusion protein-pull down assay	Mouse	732-835	Human	Not determined			20346379
TRPC6	Rab9	Fusion protein-pull down assay	Mouse	828-883	Human	Not determined			20346379
TRPC6	RNF24	Yeast two-hybrid	Not specified	3-130	Human	98-148			17850865
TRPC6	RNF24	Yeast two-hybrid	Not specified	122-259	Human	98-148			17850865
TRPC6	S100A10	Fluorescence probe labeling	Rat	801-878	Human	Not determined		310 nM	23671622
TRPC6	SNF8	Co-immunoprecipitation	Human	1-106	Human	Not determined			23171048
TRPC6	SNF8	Yeast two-hybrid	Human	1-106	Human	Not determined			23171048
TRPC6	TRPC4	Co-immunoprecipitation	Mouse	715-930	Mouse	1-304			16916799

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPC6	Calcineurin	In vivo PTM assay	Dephosphorylation			24740790
TRPC6	Calmodulin	Calcium measurement			Activation	12379180
TRPC6	Calmodulin	Patch clamp			Activation	12379180
TRPC6	FYN	In vivo PTM assay	Phosphorylation			14761972
TRPC6	FYN	Patch clamp			Activation	14761972
TRPC6	M1 mAChR	Calcium measurement			Activation	15994335
TRPC6	MxA	Calcium measurement			Activation	15757897
TRPC6	PKA	In vitro PTM assay	Phosphorylation (Thr-69)			22001259
TRPC6	PKA	In vitro PTM assay	Phosphorylation (Ser-28)			22001259
TRPC6	PKA	In vitro PTM assay	Phosphorylation (Thr-70)			23645677
TRPC6	PKA	In vivo PTM assay	Phosphorylation (Thr-70)			23645677
TRPC6	PKCơ	In vitro PTM assay	Phosphorylation (Ser-714)			15994335
TRPC6	PKG1	In vitro PTM assay	Phosphorylation (Thr-69)			18617565
TRPC6	PKG1	In vivo PTM assay	Phosphorylation (Thr-69)			24740790
TRPC6	RNF24	Cell surface biotinylation		Decrease in plasma membrane level		17850865
TRPC6	SNF8	Patch clamp			Activation	23171048
TRPC6	TRPC1	Patch clamp			Inhibition	20660561
TRPC6	TRPC5	Patch clamp			Inhibition	20660561
TRPC6	UBC	In vivo PTM assay	Ubiquitination			17110928

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPC6 / interactors