TRPM2 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily M member 2
(TRPM2)
Official Symbol: TRPM2
provided by HGNC
Function:
Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
7226 |
O94759 |
113077 |
O94759 |
494 |
hsa:7226 |
603749 |
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|
Mouse
|
28240 |
Q91YD4 |
205838 |
Q91YD4 |
mmu:28240 |
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|
Rat
|
294329 |
Q5G856 |
254675 |
Q5G856 |
rno:294329 |
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Biological Process:
- GO:0033194
: response to hydroperoxide - GO:0035725 : sodium ion transmembrane transport
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPM2 |
|
Calmodulin | Inference | Prediction | 16461353 | |||||
| TRPM2 |
|
EFHC1 | Inference | Prediction | 22226147 | |||||
| TRPM2 |
|
PKCơ | Inference | Prediction | 24337049 | |||||
| TRPM2 |
|
PTPL1 | Inference | Prediction | 17251321 | |||||
| TRPM2 |
|
Rac1 | Inference | Prediction | 25295536 | |||||
| TRPM2 |
|
UBC | Affinity purification-mass spectrometry | Not used as a bait | Human | HEK293T | Cell lystaes | 21890473 | ||
| TRPM2 |
|
UBC | Affinity purification-mass spectrometry | Not used as a bait | Human | ?HCT116 | Cell lystaes | 21906983 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | HEK293T | Human | Full-length | Not specified | Full-length | 16461353 | |
| TRPM2 |
|
EFHC1 | Co-immunofluorescence staining | HEK293 | Human | Full-length | Mouse | 319-1376 | 22226147 | |
| TRPM2 |
|
EFHC1 | Co-immunoprecipitation | Mouse brain microsome extracts | 22226147 | |||||
| TRPM2 |
|
EFHC1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Mouse | 319-1376 | 22226147 | |
| TRPM2 |
|
PKCơ | Co-immunoprecipitation | Human pulmonary arterial endothelial cell | Human | 1-846 | Not used | 24337049 | ||
| TRPM2 |
|
PTPL1 | Co-immunoprecipitation | Human monocytic U937-ecoR cell | 17251321 | |||||
| TRPM2 |
|
PTPL1 | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 17251321 | |
| TRPM2 |
|
Rac1 | Co-immunofluorescence staining | Mouse kidney section | Human | Full-length | Human | Full-length | 25295536 | |
| TRPM2 |
|
Rac1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 25295536 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 1-730 | Not specified | Not determined | 16461353 | ||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 406-416 | Not specified | Not determined | 16461353 | ||
| TRPM2 |
|
Calmodulin | Co-immunoprecipitation | Human | 1060-1503 | Not specified | Not determined | 16461353 | ||
| TRPM2 |
|
EFHC1 | Co-immunoprecipitation | Human | 1-2214 | Mouse | Not determined | 22226147 | ||
| TRPM2 |
|
EFHC1 | Co-immunoprecipitation | Human | 3169-4509 | Mouse | Not determined | 22226147 | ||
| TRPM2 |
|
PTPL1 | Fusion protein-pull down assay | Human | Not determined | Human | 1099-1199 | 17251321 | ||
| TRPM2 |
|
PTPL1 | Fusion protein-pull down assay | Human | Not determined | Human | 1888-1990 | 17251321 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPM2 |
|
Calmodulin | Calcium measurement | Activation | 16461353 | |||||||
| TRPM2 |
|
EFHC1 | Patch clamp | Activation | 22226147 | |||||||
| TRPM2 |
|
PKCơ | Calcium measurement | Activation | 24337049 | |||||||
| TRPM2 |
|
PKCơ | In vivo PTM assay | Phosphorylation (Ser-29) | 24337049 | |||||||
| TRPM2 |
|
PTPL1 | Calcium measurement | Inhibition | 17251321 | |||||||
| TRPM2 |
|
PTPL1 | In vivo PTM assay | Dephosphorylation | 17251321 | |||||||
(:
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:
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