TRPM7 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily M member 7
(TRPM7)
Official Symbol: TRPM7
provided by HGNC
Function:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
54822 |
Q96QT4 |
DIP-47486N |
MINT-2815126 |
120177 |
Q96QT4 |
499 |
hsa:54822 |
605692 |
|
|
Mouse
|
58800 |
Q923J1 |
DIP-43725N |
MINT-2524821 |
208438 |
Q923J1 |
mmu:58800 |
|||
|
Rat
|
679906 |
Q925B3 |
DIP-43727N |
MINT-2778884 |
594845 |
Q925B3 |
rno:679906 |
|||
PPI pairs:
Biological Process:
- GO:0031032
: actomyosin structure organization - GO:0016340 : calcium-dependent cell-matrix adhesion
- GO:0008219 : cell death
- GO:0046777 : protein autophosphorylation
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
- OMIM:605692
:
Amyotrophic lateral sclerosis-parkinsonism/dementia complex 1, susceptibility to
(OMIM:105500 )
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPM7 |
|
Annexin 1 | Inference | Prediction | 15485879 | |||||
| TRPM7 |
|
Ƣ-actin | Inference | Prediction | 16407977 | |||||
| TRPM7 |
|
Myosin-HIIA | Inference | Prediction | 16407977 | |||||
| TRPM7 |
|
PLCƣ1 | Inference | Prediction | 11941371 | |||||
| TRPM7 |
|
PLCƢ1 | Yeast two-hybrid | Not used as a bait | Rat | Brain | cDNA library | 11161216 | ||
| TRPM7 |
|
PLCƣ2 | Inference | Prediction | 22759789 | |||||
| TRPM7 |
|
PLCƢ2 | Inference | Prediction | 11941371 | |||||
| TRPM7 |
|
PLCƢ3 | Inference | Prediction | 11941371 | |||||
| TRPM7 |
|
PLCƤ1 | Inference | Prediction | 22180838 | |||||
| TRPM7 |
|
RacK1 | Inference | Prediction | 18258429 | |||||
| TRPM7 |
|
SNAPIN | Yeast two-hybrid | Human | 87-326 | Human | Brain | cDNA library | 17088214 | |
| TRPM7 |
|
Synapsin1 | Inference | Prediction | 17088214 | |||||
| TRPM7 |
|
Synaptotagmin 1 | Inference | Prediction | 17088214 | |||||
| TRPM7 |
|
TRPM6 | Inference | Prediction | 14976260 | |||||
| TRPM7 |
|
UBC | Affinity purification-mass spectrometry | Not used as a bait | Human | HEK293T | Cell lystaes | 21906983 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPM7 |
|
PLCƢ1 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371 |
| TRPM7 |
|
PLCƢ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838 |
| TRPM7 |
|
PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Yeast | Rat | 643-1216 | Yeast | 11941371 |
| TRPM7 |
|
PLCƢ2 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371 |
| TRPM7 |
|
PLCƢ2 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838 |
| TRPM7 |
|
PLCƢ3 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371 |
| TRPM7 |
|
PLCƢ3 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838 |
| TRPM7 |
|
PLCƤ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838 |
| TRPM7 |
|
RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | E. coli | Mouse | Full-length | In vitro translation | 18258429 |
| TRPM7 |
|
SNAPIN | Fusion protein-pull down assay | Human | 83-326 | In vitro translation | Human | Full-length | Not specified | 17088214 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPM7 |
|
Annexin 1 | Co-immunofluorescence staining | HEK293 | Human | ChaK1 domain | Human | Not determined | 24939696 | |
| TRPM7 |
|
Annexin 1 | Co-immunoprecipitation | HEK293 | Human | ChaK1 domain | Human | Not determined | 24939696 | |
| TRPM7 |
|
Ƣ-actin | Co-immunofluorescence staining | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used | 16407977 | ||
| TRPM7 |
|
Ƣ-actin | Co-immunoprecipitation | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used | 16407977 | ||
| TRPM7 |
|
Myosin-HIIA | Co-immunoprecipitation | N1E-115 neuroblastoma cell | Mouse | Full-length | Not used | 16407977 | ||
| TRPM7 |
|
Myosin-HIIA | Co-immunoprecipitation | PC12 | 16407977 | |||||
| TRPM7 |
|
PLCƣ2 | Co-immunoprecipitation | DT40 | Mouse | Full-length | Not used | 22759789 | ||
| TRPM7 |
|
PLCƢ2 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Rat | Full-length | 11941371 | |
| TRPM7 |
|
SNAPIN | Co-immunoprecipitation | Rat brain synaptic vesicle | 17088214 | |||||
| TRPM7 |
|
Synapsin1 | Co-immunofluorescence staining | Rat SCG sympathetic neuron | 17088214 | |||||
| TRPM7 |
|
Synapsin1 | Co-immunoprecipitation | Rat brain synaptic vesicle | 17088214 | |||||
| TRPM7 |
|
Synaptotagmin 1 | Co-immunoprecipitation | Rat brain synaptic vesicle | 17088214 | |||||
| TRPM7 |
|
TRPM6 | Fluorescence resonance energy transfer | HEK293 | Mouse | Full-length | Human | Full-length | 14976260 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPM7 |
|
Annexin 1 | In vitro PTM assay | Human | ChaK1 domain | Human | Not determined | 15485879 | ||
| TRPM7 |
|
Annexin 1 | In vitro PTM assay | Human | ChaK1 domain | Bovine | Not determined | 15485879 | ||
| TRPM7 |
|
Myosin-HIIA | Co-immunoprecipitation | Mouse | 1158-1864 | Mouse | 1795-1960 | 16407977 | ||
| TRPM7 |
|
PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Rat | 643-797 | 11941371 | ||
| TRPM7 |
|
RacK1 | Fusion protein-pull down assay | Mouse | ơ-kinase domain | Mouse | Not determined | 18258429 | ||
| TRPM7 |
|
SNAPIN | Fusion protein-pull down assay | Human | 87-326 | Human | 43-68 | 17088214 | ||
| TRPM7 |
|
TRPM6 | Fusion protein-pull down assay | Mouse | 1548-1863 | Mouse | 1711-2028 | 19228120 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPM7 |
|
PLCƢ1 | Patch clamp | Inhibition | 11941371 | |||||||
| TRPM7 |
|
PLCƢ2 | Patch clamp | Inhibition | 11941371 | |||||||
| TRPM7 |
|
PLCƢ3 | Patch clamp | Inhibition | 11941371 | |||||||
| TRPM7 |
|
RacK1 | Patch clamp | Inhibition | 18258429 | |||||||
| TRPM7 |
|
TRPM6 | Patch clamp | New channel creation | 24385424 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)