TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPML1 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (33)
Biological Process (3)
Disease (1)

Name: Mucolipin-1 (TRPML1)

Official Symbol: MCOLN1 provided by HGNC

Function:

Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

ALG-2/PDCD6	Cdc42	ERIGC3	HOP/STI1	HSC70	HSP40/DNAJB1	HSP40/DNAJC14	HSP90
LAPTM4a	LAPTM4b	LAPTM5	NDKA	NovelProtein/NP9	PEA-15	PEX16	PIP5K1A
PKA	Peroxisomal membrane protein 2	RFP/TRIM27	Rac1	Rac2	RhoG	SEC13	SEC31A
SLC35E1	STOML1	TMEM163	TPC1	TPC2	TRPML2	TRPML3	Unnamed protein (BAE)
YIF1B

Biological Process:

GO:0006816 : calcium ion transport
GO:0006879 : cellular iron ion homeostasis
GO:0033572 : transferrin transport

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease:

OMIM:605248 : Mucolipidosis IV (OMIM:252650 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPML1	ALG-2/PDCD6	Affinity purification-mass spectrometry	Human	1-66			HeLa lysates		19864416
TRPML1	Cdc42	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	ERIGC3	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601
TRPML1	HOP/STI1	Inference						Prediction	19117012
TRPML1	HSC70	Yeast two-hybrid	Human	94-298	Human	Brain	cDNA library		19117012
TRPML1	HSP40/DNAJB1	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	HSP40/DNAJC14	Yeast two-hybrid	Human	94-298	Human	Brain	cDNA library		19117012
TRPML1	HSP90	Inference						Prediction	19117012
TRPML1	LAPTM4a	Yeast two-hybrid	Human	Full-length	Human	adult brain	cDNA library		21224396
TRPML1	LAPTM4b	Yeast two-hybrid	Human	Full-length	Human	adult brain	cDNA library		21224396
TRPML1	LAPTM5	Speculation						Prediction	21224396
TRPML1	NDKA	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	NovelProtein/NP9	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	PEA-15	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	Peroxisomal membrane protein 2	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601
TRPML1	PEX16	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601
TRPML1	PIP5K1A	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601
TRPML1	PKA	Inference						Prediction	17988215
TRPML1	Rac1	Speculation						Prediction	23418601
TRPML1	Rac2	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	RFP/TRIM27	Yeast two-hybrid	Human	Full-length	Human	Fetal brain	cDNA library		16169070
TRPML1	RhoG	Speculation						Prediction	23418601
TRPML1	SEC13	Affinity purification-mass spectrometry	Human	1-66			HeLa lysates		19864416
TRPML1	SEC31A	Affinity purification-mass spectrometry	Human	1-66			HeLa lysates		19864416
TRPML1	SLC35E1	Yeast two-hybrid	Human	Full-length	Human	Fetal brain	cDNA library		16169070
TRPML1	STOML1	Affinity purification-mass spectrometry	Mouse	Full-length	Mouse	RAW264.7 macrophages	Lysates		23418601
TRPML1	TMEM163	Yeast two-hybrid	Mouse	Full-length	Mouse	Kidney	cDNA library		25130899
TRPML1	TPC1	Inference						Prediction	21540176
TRPML1	TPC2	Inference						Prediction	21540176
TRPML1	TRPML2	Inference						Prediction	16606612
TRPML1	TRPML3	Inference						Prediction	16606612
TRPML1	Unnamed protein (BAE)	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601
TRPML1	YIF1B	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Mouse	Heart	cDNA library		23418601

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPML1	ALG-2/PDCD6	Fusion protein-pull down assay	Human	1-66	E. coli	Human	Full-length	HeLa lysates	19864416
TRPML1	Cdc42	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	ERIGC3	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	HSC70	Fusion protein-pull down assay	Human	94-298	In vitro translation	Human	Full-length	In vitro translation	19117012
TRPML1	HSP40/DNAJC14	Fusion protein-pull down assay	Human	94-298	In vitro translation	Human	Full-length	In vitro translation	19117012
TRPML1	LAPTM4a	Yeast two-hybrid	Human	Full-length	Yeast	Human	1-651	Yeast	21224396
TRPML1	LAPTM4b	Yeast two-hybrid	Human	Full-length	Yeast	Human	79-141	Yeast	21224396
TRPML1	LAPTM5	Yeast two-hybrid	Human	Full-length	Yeast	Human	Not specified	Yeast	21224396
TRPML1	NDKA	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	NovelProtein/NP9	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	PEA-15	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	Peroxisomal membrane protein 2	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	PEX16	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	Rac1	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	Rac2	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	SEC31A	Fusion protein-pull down assay	Human	1-66	E. coli	Human	Full-length	HeLa lysates	19864416
TRPML1	Unnamed protein (BAE)	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601
TRPML1	YIF1B	Split-Ubiquitin Yeast two-hybrid	Mouse	Full-length	Yeast	Mouse	Full-length	Yeast	23418601

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPML1	ALG-2/PDCD6	Co-immunofluorescence staining	ARPE-19						19864416
TRPML1	Cdc42	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	Cdc42	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	ERIGC3	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	HOP/STI1	Co-immunoprecipitation		CHO	Human	Full-length	Not used		19117012
TRPML1	HSC70	Co-immunoprecipitation		CHO	Human	Full-length	Human	Full-length	19117012
TRPML1	HSC70	Co-immunoprecipitation		CHO	Human	Full-length	Not used		19117012
TRPML1	HSP40/DNAJC14	Co-immunofluorescence staining		CHO	Human	Full-length	Human	Full-length	19117012
TRPML1	HSP40/DNAJC14	Co-immunoprecipitation		CHO	Human	Full-length	Human	Full-length	19117012
TRPML1	HSP40/DNAJC14	Co-immunoprecipitation		CHO	Human	Full-length	Not used		19117012
TRPML1	HSP90	Co-immunoprecipitation		CHO	Human	Full-length	Not used		19117012
TRPML1	LAPTM4a	Co-immunofluorescence staining		ARPE-19	Human	Full-length	Human	Full-length	21224396
TRPML1	LAPTM4a	Co-immunoprecipitation		HeLa	Human	Full-length	Human	Full-length	21224396
TRPML1	LAPTM4b	Co-immunofluorescence staining		ARPE-19	Human	Full-length	Human	Full-length	21224396
TRPML1	LAPTM4b	Co-immunoprecipitation		HeLa	Human	Full-length	Human	Full-length	21224396
TRPML1	LAPTM5	Co-immunofluorescence staining		ARPE-19	Human	Full-length	Human	Full-length	21224396
TRPML1	LAPTM5	Co-immunoprecipitation		HeLa	Human	Full-length	Human	Full-length	21224396
TRPML1	NovelProtein/NP9	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	NovelProtein/NP9	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	PEA-15	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	PEX16	Split-Ubiquitin Yeast two-hybrid		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	PIP5K1A	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	Rac1	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	Rac2	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	RhoG	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	STOML1	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	STOML1	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	STOML1	Co-immunoprecipitation		RAW264.7 macrophage	Mouse	Full-length	Not used		23418601
TRPML1	TMEM163	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	25130899
TRPML1	TMEM163	Co-immunoprecipitation		HEK293	Human	Full-length	Mouse	Full-length	25130899
TRPML1	TPC1	Co-immunofluorescence staining		HEK293T	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC1	Co-immunofluorescence staining		HeLa	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC1	Co-immunofluorescence staining		SK-BR-3	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC1	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC2	Co-immunofluorescence staining		HEK293T	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC2	Co-immunofluorescence staining		HeLa	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC2	Co-immunofluorescence staining		SK-BR-3	Human	Full-length	Human	Full-length	21540176
TRPML1	TPC2	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	21540176
TRPML1	TRPML2	Co-immunofluorescence staining	HEK293						19557826
TRPML1	TRPML2	Co-immunofluorescence staining		CHO	Human	Full-length	Human	Full-length	19885840
TRPML1	TRPML2	Co-immunoprecipitation	HEK293						19557826
TRPML1	TRPML2	Co-immunoprecipitation		CHO	Human	Full-length	Human	Full-length	19885840
TRPML1	TRPML2	Co-immunoprecipitation		HEK293	Human	Full-length (mutation in the pore region)	Human	Full-length (mutation in the pore region)	20736310
TRPML1	TRPML2	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Human	Full-length	16606612
TRPML1	TRPML3	Co-immunofluorescence staining	HEK293						19557826
TRPML1	TRPML3	Co-immunofluorescence staining		CHO	Human	Full-length	Human	Full-length	19885840
TRPML1	TRPML3	Co-immunoprecipitation		HEK293	Not specified	Full-length	Human	Full-length	19522758
TRPML1	TRPML3	Co-immunoprecipitation	HEK293						19557826
TRPML1	TRPML3	Co-immunoprecipitation		CHO	Human	Full-length	Human	Full-length	19885840
TRPML1	TRPML3	Co-immunoprecipitation		HEK293	Human	Full-length (mutation in the pore region)	Human	Full-length (mutation in the pore region)	20736310
TRPML1	TRPML3	Fluorescence resonance energy transfer		HEK293	Not specified	Full-length	Not specified	Full-length	23469151
TRPML1	TRPML3	Fluorescence resonance energy transfer		HEK293	Human	Full-length	Mouse	Full-length	16606612
TRPML1	Unnamed protein (BAE)	Co-immunofluorescence staining		RAW264.7 macrophage	Mouse	Full-length	Mouse	Full-length	23418601
TRPML1	YIF1B	Co-immunoprecipitation		HeLa	Mouse	Full-length	Mouse	Full-length	23418601

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPML1	ALG-2/PDCD6	Fusion protein-pull down assay	Human	37-49	Human	Not determined			19864416
TRPML1	SEC31A	Fusion protein-pull down assay	Human	37-49	Human	Not determined			19864416

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPML1	PKA	In vitro PTM assay	Phosphorylation (Ser-557)			17988215
TRPML1	PKA	In vivo PTM assay	Phosphorylation (Ser-559)			17988215
TRPML1	PKA	Patch clamp			Inhibition	17988215
TRPML1	TRPML2	Patch clamp			New channel creation	19885840
TRPML1	TRPML3	Patch clamp			New channel creation	19885840

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPML1 / interactors