TRPML3 / interactors
- At a glance
- In detail
Name: Mucolipin-3
(TRPML3)
Official Symbol: MCOLN3
provided by HGNC
Function:
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
Species |
External DB
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Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
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55283![]() |
Q8TDD5![]() |
120571![]() |
Q8TDD5![]() |
503![]() |
hsa:55283![]() |
607400![]() |
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Mouse
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171166![]() |
Q8R4F0![]() |
228482![]() |
Q8R4F0![]() |
mmu:171166![]() |
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Rat
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308022![]() |
Q6AY44![]() |
DIP-39439N![]() |
Q6AY44![]() |
258885![]() |
Q6AY44![]() |
rno:308022![]() |
Biological Process:
- GO:0042491
: auditory receptor cell differentiation
- GO:0007626
: locomotory behavior
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPML3 |
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GATE16 | Yeast two-hybrid | Human | 1-312 | Mouse | Brain | cDNA library | 24269818 | |
TRPML3 |
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HSC70 | Inference | Prediction | 19117012 | |||||
TRPML3 |
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TPC1 | Inference | Prediction | 21540176 | |||||
TRPML3 |
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TPC2 | Inference | Prediction | 21540176 | |||||
TRPML3 |
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TRPML1 | Inference | Prediction | 16606612 | |||||
TRPML3 |
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TRPML2 | Inference | Prediction | 16606612 | |||||
TRPML3 |
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TRPV5 | Inference | Prediction | 23469151 | |||||
TRPML3 |
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TRPV6 | Inference | Prediction | 23469151 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | |||||||||
TRP channel construct | Interactor construct | |||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
TRPML3 |
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GATE16 | Fusion protein-pull down assay | Human | Full-length | HEK293T lysates | Mouse | Full-length | E. coli | 24269818 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPML3 |
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GATE16 | Co-immunofluorescence staining | HeLa | Human | Full-length | Mouse | Full-length | 24269818 | |
TRPML3 |
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GATE16 | Co-immunoprecipitation | HEK293T | Human | Full-length | Mouse | Full-length | 24269818 | |
TRPML3 |
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HSC70 | Co-immunoprecipitation | CHO | Human | Full-length | Not used | 19117012 | ||
TRPML3 |
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TPC1 | Co-immunofluorescence staining | HEK293T | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC1 | Co-immunofluorescence staining | HeLa | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC1 | Co-immunofluorescence staining | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC1 | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC2 | Co-immunofluorescence staining | HEK293T | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC2 | Co-immunofluorescence staining | HeLa | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC2 | Co-immunofluorescence staining | SK-BR-3 | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TPC2 | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 21540176 | |
TRPML3 |
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TRPML1 | Co-immunofluorescence staining | HEK293 | 19557826 | |||||
TRPML3 |
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TRPML1 | Co-immunofluorescence staining | CHO | Human | Full-length | Human | Full-length | 19885840 | |
TRPML3 |
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TRPML1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not specified | Full-length | 19522758 | |
TRPML3 |
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TRPML1 | Co-immunoprecipitation | HEK293 | 19557826 | |||||
TRPML3 |
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TRPML1 | Co-immunoprecipitation | CHO | Human | Full-length | Human | Full-length | 19885840 | |
TRPML3 |
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TRPML1 | Co-immunoprecipitation | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310 | |
TRPML3 |
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TRPML1 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151 | |
TRPML3 |
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TRPML1 | Fluorescence resonance energy transfer | HEK293 | Mouse | Full-length | Human | Full-length | 16606612 | |
TRPML3 |
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TRPML2 | Co-immunofluorescence staining | HEK293 | 19557826 | |||||
TRPML3 |
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TRPML2 | Co-immunoprecipitation | HEK293 | 19557826 | |||||
TRPML3 |
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TRPML2 | Co-immunoprecipitation | HEK293 | Human | Full-length (mutation in the pore region) | Human | Full-length (mutation in the pore region) | 20736310 | |
TRPML3 |
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TRPML2 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151 | |
TRPML3 |
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TRPML2 | Fluorescence resonance energy transfer | HEK293 | Mouse | Full-length | Human | Full-length | 16606612 | |
TRPML3 |
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TRPV5 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Mouse | Full-length | 23469151 | |
TRPML3 |
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TRPV6 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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---|---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPML3 |
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GATE16 | Co-immunoprecipitation | Human | 1-312 | Mouse | Not determined | 24269818 | ||
TRPML3 |
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GATE16 | Fusion protein-pull down assay | Human | 1-312 | Mouse | Not determined | 24269818 | ||
TRPML3 |
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TRPV5 | Patch clamp | 1:1 | 23469151 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPML3 |
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TRPML1 | Co-immunofluorescence staining | Increase in lysosomal membrane level | 16606612 | |||||||
TRPML3 |
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TRPML1 | Patch clamp | New channel creation | 19885840 | |||||||
TRPML3 |
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TRPML2 | Co-immunofluorescence staining | Increase in lysosomal membrane level | 16606612 | |||||||
TRPML3 |
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TRPV5 | Patch clamp | New channel creation | 23469151 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
