TRPP2 / interactors
- At a glance
- In detail
Name: Polycystic kidney disease 2-like 1 protein
(TRPP2)
Official Symbol: PKD2L1
provided by HGNC
Function:
May function as a subunit of an ion channel and act as a transducer of calcium-mediated signaling.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
9033 |
Q9P0L9 |
MINT-3973148 |
114499 |
Q9P0L9 |
505 |
hsa:9033 |
604532 |
||
|
Mouse
|
329064 |
A2A259 |
236700 |
mmu:329064 |
||||||
|
Rat
|
293937 |
D3ZDT6 |
254514 |
rno:293937 |
||||||
PPI pairs:
Biological Process:
- GO:0071468
: cellular response to acidity - GO:0001581 : detection of chemical stimulus involved in sensory perception of sour taste
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPP2 |
|
ơ-actinin | Inference | Prediction | 17944866 | |||||
| TRPP2 |
|
ơ-actinin-1 | Inference | Prediction | 17944866 | |||||
| TRPP2 |
|
ơ-actinin-2 | Inference | Prediction | 17944866 | |||||
| TRPP2 |
|
PKD1/Polycystin-1 | Inference | Prediction | 15548533 | |||||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Inference | Prediction | 16891422 | |||||
| TRPP2 |
|
Troponin I 1 | Inference | Prediction | 12809519 | |||||
| TRPP2 |
|
Troponin I 2 | Inference | Prediction | 12809519 | |||||
| TRPP2 |
|
Troponin I 3 | Inference | Prediction | 12809519 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPP2 |
|
ơ-actinin | Fusion protein-pull down assay | Human | 1-97 | E. coli | Chicken | Full-length | In vitro translation | 17944866 |
| TRPP2 |
|
ơ-actinin | Fusion protein-pull down assay | Human | 562-805 | E. coli | Chicken | Full-length | In vitro translation | 17944866 |
| TRPP2 |
|
ơ-actinin-1 | Yeast two-hybrid | Human | 1-97 | Yeast | Human | 334-892 | Yeast | 17944866 |
| TRPP2 |
|
ơ-actinin-1 | Yeast two-hybrid | Human | 562-805 | Yeast | Human | 334-892 | Yeast | 17944866 |
| TRPP2 |
|
ơ-actinin-2 | Yeast two-hybrid | Human | 1-97 | Yeast | Human | 341-894 | Yeast | 17944866 |
| TRPP2 |
|
ơ-actinin-2 | Yeast two-hybrid | Human | 562-805 | Yeast | Human | 341-894 | Yeast | 17944866 |
| TRPP2 |
|
Troponin I 1 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519 |
| TRPP2 |
|
Troponin I 2 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519 |
| TRPP2 |
|
Troponin I 3 | Fusion protein-pull down assay | Human | 562-805 | E. coli | Human | Not specified | Not specified | 12809519 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPP2 |
|
ơ-actinin-1 | Co-immunoprecipitation | HEK293 | 17944866 | |||||
| TRPP2 |
|
ơ-actinin-2 | Co-immunoprecipitation | Mouse brain lysates | 17944866 | |||||
| TRPP2 |
|
PKD1/Polycystin-1 | Co-immunoprecipitation | mIMCD-3 | 16609150 | |||||
| TRPP2 |
|
PKD1/Polycystin-1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 15548533 | |
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunofluorescence staining | Mouse taste cell | 16891422 | |||||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | HEK293T | Mouse | Full-length | Mouse | Full-length | 16891422 | |
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | HEK293T | Mouse | Full-length | Mouse | Full-length | 20538909 | |
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Human | Full-length | 23212381 | |
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | HEK293T | Mouse | Full-length | Mouse | Full-length | 18535624 | |
| TRPP2 |
|
Troponin I 1 | Co-immunoprecipitation | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 1 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 2 | Co-immunoprecipitation | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 2 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 3 | Co-immunoprecipitation | NIH-3T3 | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 3 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Human | Full-length | 12809519 | |
| TRPP2 |
|
Troponin I 3 | Co-immunoprecipitation | Human adult cardiac tissue | 12809519 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPP2 |
|
ơ-actinin-2 | Yeast two-hybrid | Human | 1-97 | Human | 596-728 | 17944866 | ||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | Mouse | Full-length | Mouse | 1594-2151 | 16891422 | ||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunoprecipitation | Mouse | Not determined | Mouse | 1896-2040 | 20538909 | ||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Single-molecule photobleaching | 3:1 | 23212381 | |||||
| TRPP2 |
|
Troponin I 3 | Yeast two-hybrid | Human | 630-705 | Human | Not determined | 12809519 | ||
| TRPP2 |
|
Troponin I 3 | Yeast two-hybrid | Human | 562-805 | Human | 1-170 | 12809519 | ||
| TRPP2 |
|
Troponin I 3 | Yeast two-hybrid | Human | 562-805 | Human | 106-158 | 12809519 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPP2 |
|
ơ-actinin | Patch clamp | Activation | 17944866 | |||||||
| TRPP2 |
|
PKD1/Polycystin-1 | Co-immunofluorescence staining | Increase in plasma membrane level | 16609150 | |||||||
| TRPP2 |
|
PKD1/Polycystin-1 | Co-immunofluorescence staining | Increase in plasma membrane level | 15548533 | |||||||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Co-immunofluorescence staining | Increase in plasma membrane level | 20538909 | |||||||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Patch clamp | New channel creation | 23212381 | |||||||
| TRPP2 |
|
PKD1L3/Polycystin-1L3 | Patch clamp | New channel creation | 18535624 | |||||||
| TRPP2 |
|
Troponin I 1 | Calcium measurement | Inhibition | 12809519 | |||||||
| TRPP2 |
|
Troponin I 1 | Patch clamp | Inhibition | 12809519 | |||||||
| TRPP2 |
|
Troponin I 3 | Calcium measurement | Inhibition | 12809519 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)