TRPP3 / interactors
- At a glance
- In detail
Name: Polycystic kidney disease 2-like 2 protein
(TRPP3)
Official Symbol: PKD2L2
provided by HGNC
Function:
May function as a subunit of a cation channel and play a role in fertilization.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
Species |
External DB
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Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
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27039![]() |
Q9NZM6![]() |
117970![]() |
Q9NZM6![]() |
506![]() |
hsa:27039![]() |
604669![]() |
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Mouse
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53871![]() |
Q9JLG4![]() |
207500![]() |
Q9JLG4![]() |
mmu:53871![]() |
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Rat
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291683![]() |
D4A828![]() |
253607![]() |
rno:291683![]() |
PPI pairs:
Biological Process:
No information in Gene Ontology
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPP3 |
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RACK1 | Yeast two-hybrid | Human | 1-291 | Human | Kidney | cDNA library | 22174419 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
![Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4](/assets/link-2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4.gif)
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Validation: In vitro validation
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Assay with recombinant proteins | Reference | |||||||||
TRP channel construct | Interactor construct | |||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
TRPP3 |
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RACK1 | Fusion protein-pull down assay | Human | 1-291 | HEK293 lysates | Human | Full-length | E. coli | 22174419 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
![Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4](/assets/link-2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4.gif)
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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPP3 |
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RACK1 | Co-immunoprecipitation | Xenopus oocyte | Human | Full-length | Human | Full-length | 22174419 | |
TRPP3 |
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RACK1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 22174419 | |
TRPP3 |
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RACK1 | Co-immunoprecipitation | mIMCD-3 | Human | Full-length | Human | Full-length | 22174419 | |
TRPP3 |
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RACK1 | Co-immunoprecipitation | Mouse kidney lysates | 22174419 | |||||
TRPP3 |
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RACK1 | Co-immunoprecipitation | Mouse heart tissue lysates | 22174419 | |||||
TRPP3 |
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RACK1 | Co-immunoprecipitation | Mouse brain tissue lysates | 22174419 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
![Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4](/assets/link-2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4.gif)
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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPP3 |
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RACK1 | Yeast two-hybrid | Human | 1-291 | Human | 415-669 | 22174419 | ||
TRPP3 |
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RACK1 | Yeast two-hybrid | Human | 1-291 | Human | 649-951 | 22174419 | ||
TRPP3 |
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RACK1 | Yeast two-hybrid | Human | 55-291 | Human | 415-951 | 22174419 | ||
TRPP3 |
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RACK1 | Yeast two-hybrid | Human | 1696-2415 | Human | Not specified | 22174419 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
![Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4](/assets/link-2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4.gif)
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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPP3 |
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RACK1 | Calcium measurement | Inhibition | 22174419 | |||||||
TRPP3 |
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RACK1 | Cell surface biotinylation | Decrease in plasma membrane level | 22174419 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
![Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4](/assets/link-2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4.gif)