TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPV1 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (94)
Biological Process (3)
Disease (0)

Name: Transient receptor potential cation channel subfamily V member 1 (TRPV1)

Official Symbol: TRPV1 provided by HGNC

Function:

Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity).

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

80K-H	AKAP5/AKAP150	AMPKơ2	Actin	Akt	Apolipoprotein O	Bkca	CB1
CDK12	CDK5	CHERP	CaMKII	Calmodulin	Catenin-ơ2	Catenin-Ƥ2	Cbl
Contactin-1	DAP-150	DAZAP2	E-cadherin	EGFR	Eferin/Rab11-FIP3	FAF1	GABARAP
GCN5	Glucose 6 phosphatase	Glycogen phosphorylase	HAX-1	ITM2C	JMJD2C	KCNQ2	KCNQ3
KIF13B	KIF2A	KIF3B	KPNƢ1	KvƢ2	NKX2.2	NMDAR1	NMDAR2B
NOMO1	Neuronatin	OS-9	P2RX3	PACS-1	PEX10	PI3K-p85ơ	PI3K-p85Ƣ
PIRT	PKA	PKARIIơ	PKC	PKCƢ	PKCƥ	PLAGL1	PLCƣ1
POLR2G	POLR3D	PPARơ	PRKD1/PKCƬ	RAB26	RAB8b	RANBP9	RPL12
Ribosomal protein L9	RyR1	SGT	SMA3	SNAPIN	Src	Synaptotagmin 1	Synaptotagmin 4
TAK1	TGS1	TMEM115	TRPA1	TRPV2	TRPV3	Thrombospondin-3	TrkA
Tubulin	UBP5	ZBTB17	ZO-1	eNOS	snRNP 70	ơ-tubulin-1A	ơ-tubulin-1B
ơENaC	ơII-spectrin	Ƣ-arrestin 2	Ƣ-catenin	Ƣ-tubulin-3	Ƣ-tubulin-5

Biological Process:

GO:0007166 : cell surface receptor signaling pathway
GO:0007635 : chemosensory behavior
GO:0050955 : thermoception

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease: No information in OMIM

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPV1	80K-H	Speculation						Prediction	19801576
TRPV1	Actin	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	AKAP5/AKAP150	Inference						Prediction	18381233
TRPV1	Akt	Inference						Prediction	21493704
TRPV1	AMPKơ2	Inference						Prediction	22451268
TRPV1	Apolipoprotein O	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	BKca	Inference						Prediction	24147119
TRPV1	Calmodulin	Inference						Prediction	12808128
TRPV1	CaMKII	Inference						Prediction	21493704
TRPV1	Catenin-ơ2	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	Catenin-Ƥ2	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	CB1	Inference						Prediction	23142606
TRPV1	Cbl	Inference						Prediction	19155296
TRPV1	CDK12	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	CDK5	Inference						Prediction	17194758
TRPV1	CHERP	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	Contactin-1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	DAP-150	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	DAZAP2	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	E-cadherin	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	E-cadherin	Inference						Prediction	23345400
TRPV1	Eferin/Rab11-FIP3	Yeast two-hybrid	Rat	684-820	Mouse	Brain	cDNA library		15883036
TRPV1	EGFR	Inference						Prediction	19155296
TRPV1	eNOS	Inference						Prediction	21493704
TRPV1	FAF1	Yeast two-hybrid	Rat	243-440	Rat	Sensory neuron	cDNA expression library		16510717
TRPV1	GABARAP	Yeast two-hybrid	Rat	1-414	Rat	Brain	cDNA library		20179142
TRPV1	GCN5	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	Glucose 6 phosphatase	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	Glycogen phosphorylase	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	Ƣ-arrestin 2	Speculation						Prediction	22952227
TRPV1	Ƣ-catenin	Inference						Prediction	23345400
TRPV1	Ƣ-tubulin-3	Inference						Prediction	17298389
TRPV1	Ƣ-tubulin-5	Affinity purification-mass spectrometry	Rat	681-838	Rat	Spinal cord	Extract		15569253
TRPV1	HAX-1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	ITM2C	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	JMJD2C	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	KCNQ2	Inference						Prediction	21844219
TRPV1	KCNQ3	Inference						Prediction	21844219
TRPV1	KIF13B	Inference						Prediction	23077056
TRPV1	KIF2A	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	KIF3B	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	KPNƢ1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	KvƢ2	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	Neuronatin	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	NKX2.2	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	NMDAR1	Inference						Prediction	22609428
TRPV1	NMDAR2B	Inference						Prediction	22609428
TRPV1	NOMO1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	ơ-tubulin-1A	Inference						Prediction	15569253
TRPV1	ơ-tubulin-1B	Inference						Prediction	22262838
TRPV1	ơENaC	Inference						Prediction	24890824
TRPV1	ơII-spectrin	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	OS-9	Inference						Prediction	17932042
TRPV1	P2RX3	Inference						Prediction	19223122
TRPV1	PACS-1	Inference						Prediction	23159249
TRPV1	PEX10	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	PI3K-p85Ƣ	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	PI3K-p85ơ	Inference						Prediction	17074976
TRPV1	PIRT	Inference						Prediction	18455988
TRPV1	PKA	Inference						Prediction	12194871
TRPV1	PKARIIơ	Inference						Prediction	18463244
TRPV1	PKC	Inference						Prediction	14523239
TRPV1	PKCƢ	Inference						Prediction	24920628
TRPV1	PKCƥ	Inference						Prediction	11884385
TRPV1	PLAGL1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	PLCƣ1	Inference						Prediction	15744307
TRPV1	POLR2G	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	POLR3D	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	PPARơ	Inference						Prediction	25014183
TRPV1	PRKD1/PKCƬ	Inference						Prediction	15471852
TRPV1	RAB26	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	RAB8b	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	RANBP9	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	Ribosomal protein L9	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	RPL12	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	RyR1	Affinity purification-mass spectrometry	Not used as a bait		Rabbit	Fast-twitch muscle	Triad vesicles		18215135
TRPV1	SGT	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	SMA3	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	SNAPIN	Yeast two-hybrid	Rat	1-414	Rat	Brain	cDNA library		15066994
TRPV1	SNAPIN	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	snRNP 70	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	SRC	Inference						Prediction	15084474
TRPV1	Synaptotagmin 1	Inference						Prediction	15066994
TRPV1	Synaptotagmin 4	Yeast two-hybrid	Rat	1-414	Rat	Brain	cDNA library		15066994
TRPV1	TAK1	Inference						Prediction	23142606
TRPV1	TGS1	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	Thrombospondin-3	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	TMEM115	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	TrkA	Inference						Prediction	11418861
TRPV1	TRPA1	Inference						Prediction	20231274
TRPV1	TRPV2	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102
TRPV1	TRPV2	Inference						Prediction	15713749
TRPV1	TRPV3	Inference						Prediction	12077606
TRPV1	UBP5	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	ZBTB17	Yeast two-hybrid	Rat	1-432	Human	Fetal brain	cDNA library		17074976
TRPV1	ZO-1	Affinity purification-mass spectrometry	Not used		Mouse	Dorsal root ganglion tissue	Lysates		24036102

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPV1	AKAP5/AKAP150	Fusion protein-pull down assay	Human	681-839	E. coli	Not used		HEK293 lysates	18701070
TRPV1	Calmodulin	Fast performance liquid chromatography	Rat	759-802	E. coli	Human	Full-length	E. coli	23109716
TRPV1	Calmodulin	Fast performance liquid chromatography	Rat	712?838	E. coli	Mouse	Full-length	E. coli	23119017
TRPV1	Calmodulin	Fusion protein-pull down assay	Rat	N-terminus	E. coli	Not specified	Full-length	In vitro translation	14699077
TRPV1	Calmodulin	Fusion protein-pull down assay	Rat	C-terminus	E. coli	Bovine	Full-length	Not specified	12808128
TRPV1	Calmodulin	X-ray diffraction-structural analysis	Rat	784-798	E. coli	Human	Full-length	E. coli	23109716
TRPV1	Cbl	Fusion protein-pull down assay	Not specified	680-838	HEK293 lysates	Not used		A431 Human epithelial carcinoma cell line	19155296
TRPV1	Eferin/Rab11-FIP3	Fusion protein-pull down assay	Rat	Full-length	HEK293T lysates	Mouse	Full-length	E. coli	15883036
TRPV1	EGFR	Fusion protein-pull down assay	Not specified	680-838	HEK293 lysates	Not used		A431 Human epithelial carcinoma cell line	19155296
TRPV1	FAF1	Fusion protein-pull down assay	Rat	1-440	E. coli	Rat	Full-length	E. coli	16510717
TRPV1	GABARAP	Fusion protein-pull down assay	Not used		Rat brain lysates	Human	Full-length	E. coli	20179142
TRPV1	Ƣ-tubulin-3	Fusion protein-pull down assay	Rat	681-838	E. coli	Not used		Pig brain tissue lysates	17298389
TRPV1	Ƣ-tubulin-5	Fusion protein-pull down assay	Rat	681-838	E. coli	Rat	Full-length	Rat spinal cord extract	15569253
TRPV1	KIF13B	Co-immunoprecipitation	Rat	Full-length	CHO cell lysates	Rat	1-557	E. coli	23077056
TRPV1	ơ-tubulin-1A	Fusion protein-pull down assay	Rat	681-838	E. coli	Rat	Full-length	Rat spinal cord extract	15569253
TRPV1	PI3K-p85ơ	Fusion protein-pull down assay	Rat	Full-length	HEK293 lysates	Not specified	Full-length	E. coli	17074976
TRPV1	PLCƣ1	Yeast two-hybrid	Rat	761-835	Yeast	Rat	796-920	Yeast	15744307
TRPV1	PRKD1/PKCƬ	Fusion protein-pull down assay	Rat	Full-length	E. coli	Not specified	Full-length	Not specified	15471852
TRPV1	SNAPIN	Fusion protein-pull down assay	Rat	1-414	E. coli	Human	Full-length	In vitro translation	15066994
TRPV1	Synaptotagmin 1	Fusion protein-pull down assay	Rat	1-414	E. coli	Human	Full-length	In vitro translation	15066994
TRPV1	Synaptotagmin 4	Fusion protein-pull down assay	Rat	1-414	E. coli	Human	Full-length	In vitro translation	15066994

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPV1	80K-H	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	19801576
TRPV1	AKAP5/AKAP150	Co-immunofluorescence staining	Rat trigeminal ganglia neuron						18381233
TRPV1	AKAP5/AKAP150	Co-immunofluorescence staining	Mouse dorsal root ganglion tissue						18463244
TRPV1	AKAP5/AKAP150	Co-immunofluorescence staining	Rat dorsal root ganglion neuron						18701070
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Rat trigeminal ganglia neuron						22789851
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Rat trigeminal ganglia neuron						18381233
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation		CHO	Rat	Full-length	Rat	Full-length	18381233
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Mouse spinalcord tissue						18463244
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Mouse dorsal root ganglion tissue						18463244
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	23264624
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation		HEK293	Human	Full-length	Mouse	Full-length	23264624
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Rat dorsal root ganglion neuron						18701070
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		18701070
TRPV1	Akt	Co-immunoprecipitation	Bovine aortic endothelial cells						21493704
TRPV1	AMPKơ2	Co-immunoprecipitation	Bovine aortic endothelial cells						22451268
TRPV1	BKca	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Human	Full-length	24147119
TRPV1	BKca	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	24147119
TRPV1	BKca	Co-immunoprecipitation	Rat dorsal root ganglion neuron						24147119
TRPV1	Calmodulin	Co-immunoprecipitation		HEK293	Rat	Full-length	Rat	Full-length	12808128
TRPV1	CaMKII	Co-immunoprecipitation	Bovine aortic endothelial cells						21493704
TRPV1	CB1	Co-immunofluorescence staining	Mouse corneal epithelium section						23142606
TRPV1	CB1	Co-immunofluorescence staining	Human corneal epithelial cells						23142606
TRPV1	CB1	Co-immunoprecipitation	Human corneal epithelial cells						23142606
TRPV1	E-cadherin	Co-immunofluorescence staining	Rat SMG cell line (SMG-C6)						23345400
TRPV1	E-cadherin	Co-immunoprecipitation	Rat SMG cell line (SMG-C6)						23345400
TRPV1	Eferin/Rab11-FIP3	Co-immunofluorescence staining		HEK293T	Rat	Full-length	Human	Full-length	15883036
TRPV1	Eferin/Rab11-FIP3	Co-immunoprecipitation		HEK293T	Rat	Full-length	Human	Full-length	15883036
TRPV1	EGFR	Co-immunoprecipitation		HEK293	Not specified	Full-length	Not used		19155296
TRPV1	EGFR	Co-immunoprecipitation	HaCaT						19155296
TRPV1	eNOS	Co-immunoprecipitation	Bovine aortic endothelial cells						21493704
TRPV1	eNOS	Co-immunoprecipitation	Bovine aortic endothelial cells						22451268
TRPV1	eNOS	Mammalian two-hybrid		Bovine aortic endothelial cells	Rat	Full-length	Human	Full-length	22451268
TRPV1	FAF1	Co-immunofluorescence staining	Rat dorsal root ganglion cell						16510717
TRPV1	FAF1	Co-immunoprecipitation	Rat dorsal root ganglion cell						16510717
TRPV1	FAF1	Co-immunoprecipitation		HEK293T	Rat	Full-length	Rat	Full-length	16510717
TRPV1	GABARAP	Co-immunofluorescence staining	Neonatal rat dorsal root ganglion						20179142
TRPV1	GABARAP	Fluorescence resonance energy transfer		HEK293	Rat	Full-length	Human	Full-length	20179142
TRPV1	Ƣ-arrestin 2	Co-immunoprecipitation	Rat trigeminal ganglia sensory neuron						22952227
TRPV1	Ƣ-arrestin 2	Co-immunoprecipitation	Rat trigeminal (TG) ganglia						24695785
TRPV1	Ƣ-arrestin 2	Co-immunoprecipitation		CHO	Rat	Full-length	Bovine	Full-length	23360390
TRPV1	Ƣ-catenin	Co-immunofluorescence staining	Rat SMG cell line (SMG-C6)						23345400
TRPV1	Ƣ-catenin	Co-immunoprecipitation	Rat SMG cell line (SMG-C6)						23345400
TRPV1	Ƣ-tubulin-5	Co-immunoprecipitation		Rat dorsal root ganglion derived cell line F11	Rat	Full-length	Not used		15569253
TRPV1	KCNQ2	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	21844219
TRPV1	KCNQ3	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	21844219
TRPV1	KIF13B	Co-immunoprecipitation		CHO (rTRPV1 stable cell)	Not used		Rat	1-557	23077056
TRPV1	KIF13B	Co-immunoprecipitation		F11	Rat	Full-length	Rat	1-557	23077056
TRPV1	KvƢ2	Co-immunofluorescence staining	Mouse dorsal root ganglion neuron						24036102
TRPV1	KvƢ2	Co-immunofluorescence staining		HEK293 tsA 201	Mouse	Full-length	Not specified	Not specified	24036102
TRPV1	KvƢ2	Co-immunoprecipitation		HEK293 tsA 201	Mouse	Full-length	Not specified	Not specified	24036102
TRPV1	NMDAR1	Co-immunofluorescence staining	Rat trigeminal ganglia lysates						22609428
TRPV1	NMDAR1	Co-immunoprecipitation	Rat trigeminal ganglia lysates						22609428
TRPV1	NMDAR2B	Co-immunoprecipitation	Rat trigeminal ganglia lysates						22609428
TRPV1	ơ-tubulin-1B	Fluorescence resonance energy transfer		CHO	Mouse	Full-length	Human	Full-length	22262838
TRPV1	ơENaC	Co-immunofluorescence staining	Mouse M1-cortical collecting duct						24890824
TRPV1	ơENaC	Co-immunoprecipitation	Mouse M1-cortical collecting duct						24890824
TRPV1	OS-9	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Mouse	Full-length	17932042
TRPV1	P2RX3	Co-immunoprecipitation		HEK293	Rat	Full-length	Human	Full-length	19223122
TRPV1	PACS-1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	23159249
TRPV1	PI3K-p85Ƣ	Co-immunoprecipitation		HEK293	Rat	Full-length	Not used		17074976
TRPV1	PI3K-p85Ƣ	Co-immunoprecipitation	Mouse dorsal root ganglion cell						17074976
TRPV1	PIRT	Co-immunoprecipitation		HEK293	Rat	Full-length	Mouse	Full-length	18455988
TRPV1	PKARIIơ	Co-immunoprecipitation	Mouse spinalcord tissue						18463244
TRPV1	PKCƢ	Co-immunofluorescence staining		HEK293	Human	Full-length	Human	Full-length	24920628
TRPV1	PKCƢ	Co-immunofluorescence staining	Rat dorsal root ganglion cell						24920628
TRPV1	PKCƢ	Co-immunoprecipitation	Rat dorsal root ganglion cell						24920628
TRPV1	PKCƢ	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	24920628
TRPV1	PPARơ	Co-immunoprecipitation		CHO	Rat	Full-length	Rat	Full-length	25014183
TRPV1	PRKD1/PKCƬ	Co-immunofluorescence staining	Rat dorsal root ganglion section						18063480
TRPV1	PRKD1/PKCƬ	Co-immunoprecipitation	Rat dorsal root ganglion lysates						18063480
TRPV1	PRKD1/PKCƬ	Co-immunoprecipitation		CHO	Rat	Full-length	Human	Full-length	15471852
TRPV1	PRKD1/PKCƬ	Co-immunoprecipitation	Rat dorsal root ganglion neuron						15471852
TRPV1	SNAPIN	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	15066994
TRPV1	SRC	Co-immunoprecipitation	Rat dorsal root ganglion neuron						15084474
TRPV1	SRC	Co-immunoprecipitation	HEK293						16319926
TRPV1	Synaptotagmin 1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	15066994
TRPV1	Synaptotagmin 4	Co-immunofluorescence staining	Rat dorsal root ganglion neuron						15066994
TRPV1	Synaptotagmin 4	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	15066994
TRPV1	TAK1	Co-immunoprecipitation	Human corneal epithelial cells						23142606
TRPV1	TrkA	Co-immunoprecipitation		HEK293	Rat	Full-length	Rat	Full-length	11418861
TRPV1	TRPA1	Co-immunoprecipitation		CHO	Rat	Full-length	Mouse	Full-length	20231274
TRPV1	TRPA1	Co-immunoprecipitation	Mouse trigeminal ganglia lysates						20231274
TRPV1	TRPV2	Co-immunofluorescence staining	Rat dorsal root ganglion tissue						16115206
TRPV1	TRPV2	Co-immunofluorescence staining		HEK293	Rat	Full-length	Rat	Full-length	16237318
TRPV1	TRPV2	Co-immunofluorescence staining	Rat dorsal root ganglion section						16237318
TRPV1	TRPV2	Co-immunoprecipitation		HEK293	Rat	Full-length	Rat	Full-length	16115206
TRPV1	TRPV2	Co-immunoprecipitation	Rat brain cortical lysates						16115206
TRPV1	TRPV2	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	16237318
TRPV1	TRPV2	Co-immunoprecipitation		Rat dorsal root ganglion derived cell line F11	Rat	Full-length	Rat	Full-length	16237318
TRPV1	TRPV2	Co-immunoprecipitation	Rat dorsal root ganglion lysates						16237318
TRPV1	TRPV2	Co-immunoprecipitation	Rat spinal cord lysates						16237318
TRPV1	TRPV2	Co-immunoprecipitation		HEK293	Rat	Full-length	Mouse	Full-length	15713749
TRPV1	TRPV3	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	12077606
TRPV1	Tubulin	Co-immunofluorescence staining	Rat supraoptic nucleus lysates						25123313
TRPV1	Tubulin	Co-immunoprecipitation	Rat supraoptic nucleus lysates						25123313

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Human	Not determined	Human	326-336			23699529
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation	Human	736-749	Human	Not determined			18701070
TRPV1	AKAP5/AKAP150	Fluorescence resonance energy transfer	Human	Not determined	Human	326-336			23699529
TRPV1	AKAP5/AKAP150	Fusion protein-pull down assay	Human	736-749	Human	Not determined			18701070
TRPV1	AKAP5/AKAP150	Patch clamp	Human	738 ?745	Human	Not determined			23616546
TRPV1	Calmodulin	Fast performance liquid chromatography	Rat	712?838	Mouse	Not determined			23119017
TRPV1	Calmodulin	Fusion protein-pull down assay	Rat	767-801	Bovine	Not determined			12808128
TRPV1	Calmodulin	Fusion protein-pull down assay	Human	101-364	Not specified	Not determined			19864432
TRPV1	Calmodulin	Fusion protein-pull down assay	Rat	189-222	Not specified	Not determined			14699077
TRPV1	Calmodulin	Fusion protein-pull down assay	Rat	ARD domain	Not specified	Not determined			20926660
TRPV1	Calmodulin	X-ray diffraction-structural analysis	Rat	784-798	Human	11-145			23109716
TRPV1	Eferin/Rab11-FIP3	Yeast two-hybrid	Rat	684-820	Mouse	345-444			15883036
TRPV1	FAF1	Fusion protein-pull down assay	Rat	243-440	Rat	110-169			16510717
TRPV1	GABARAP	Fusion protein-pull down assay	Rat	1-414	Human	1-36			20179142
TRPV1	Ƣ-tubulin-3	Fusion protein-pull down assay	Rat	681-730	Pig	Not determined			17298389
TRPV1	KCNQ2	Co-immunoprecipitation	Human	Not determined	Human	97-318			21844219
TRPV1	KCNQ2	Co-immunoprecipitation	Human	1-684	Human	Not determined			21844219
TRPV1	KIF13B	Cell surface biotinylation	Rat	Not determined	Rat	455-557			23077056
TRPV1	KIF13B	Co-immunoprecipitation	Rat	Not determined	Rat	455-557			23077056
TRPV1	PI3K-p85ơ	Fusion protein-pull down assay	Rat	N-terminus	Not specified	SH2 domain			17074976
TRPV1	PIRT	Co-immunoprecipitation	Rat	Not determined	Mouse	110-135			18455988
TRPV1	PKCƢ	Fusion protein-pull down assay	Human	1-433	Human	341-673			24920628
TRPV1	PLCƣ1	Yeast two-hybrid	Rat	761-835	Rat	861-920			15744307
TRPV1	PRKD1/PKCƬ	Fusion protein-pull down assay	Rat	99-118	Human	Not determined			15471852
TRPV1	SRC	Fusion protein-pull down assay	Human	1-433	Rat	83-144			16319926
TRPV1	SRC	Fusion protein-pull down assay	Human	681-839	Rat	83-144			16319926
TRPV1	Synaptotagmin 4	Fusion protein-pull down assay	Rat	1-414	Human	C2A domain			15066994

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPV1	AKAP5/AKAP150	Cell surface biotinylation		Increase in plasma membrane level		18701070
TRPV1	AKAP5/AKAP150	Co-immunofluorescence staining		Increase in plasma membrane level		23699529
TRPV1	AKAP5/AKAP150	Co-immunoprecipitation		Increase in plasma membrane level		23616546
TRPV1	AKAP5/AKAP150	In vivo PTM assay	Phosphorylation			18381233
TRPV1	AKAP5/AKAP150	Patch clamp			Activation	18701070
TRPV1	Calmodulin	Patch clamp			Inhibition	14699077
TRPV1	Calmodulin	Patch clamp			Sensitization	23109716
TRPV1	Calmodulin	Patch clamp			Inhibition	12808128
TRPV1	CB1	Patch clamp			Inhibition	23142606
TRPV1	CDK5	Cell surface biotinylation		Increase in plasma membrane level		23077056
TRPV1	CDK5	In vitro PTM assay	Phosphorylation (Thr-407)			17194758
TRPV1	CDK5	In vitro PTM assay	Phosphorylation			22451679
TRPV1	CDK5	In vivo PTM assay	Phosphorylation (Thr-407)			17194758
TRPV1	FAF1	Calcium measurement			Inhibition	16510717
TRPV1	FAF1	Patch clamp			Inhibition	16510717
TRPV1	GABARAP	Calcium measurement			Inhibition	20179142
TRPV1	GABARAP	Cell surface biotinylation		Increase in plasma membrane level		20179142
TRPV1	GABARAP	Patch clamp			Inhibition	20179142
TRPV1	Ƣ-arrestin 2	Calcium measurement			Desensitization	22952227
TRPV1	Ƣ-arrestin 2	In vivo PTM assay	Dephosphorylation			22952227
TRPV1	KIF13B	Cell surface biotinylation		Increase in plasma membrane level		23077056
TRPV1	KvƢ2	Cell surface biotinylation		Increase in plasma membrane level		24036102
TRPV1	PIRT	Patch clamp			Activation	18455988
TRPV1	PKA	In vitro PTM assay	Phosphorylation (Ser-116)			12194871
TRPV1	PKA	Patch clamp			Activation	12194871
TRPV1	PKC	In vitro PTM assay	Phosphorylation			14523239
TRPV1	PKCƢ	In vivo PTM assay	Phosphorylation (Thr-705)			24920628
TRPV1	PKCƢ	Patch clamp			Activation	24920628
TRPV1	PKCƥ	In vitro PTM assay	Phosphorylation (Ser-800)			11884385
TRPV1	PKCƥ	In vivo PTM assay	Phosphorylation (Ser-502)			11884385
TRPV1	PKCƥ	Patch clamp			Activation	11884385
TRPV1	PPARơ	Calcium measurement			Activation	25014183
TRPV1	PPARơ	Patch clamp			Activation	25014183
TRPV1	PRKD1/PKCƬ	Calcium measurement			Activation	15471852
TRPV1	PRKD1/PKCƬ	In vitro PTM assay	Phosphorylation (Ser-116)			15471852
TRPV1	SRC	Calcium measurement			Activation	16319926
TRPV1	SRC	In vitro PTM assay	Phosphorylayion (Tyr-200)			16319926
TRPV1	SRC	In vivo PTM assay	Phosphorylation			15084474
TRPV1	SRC	In vivo PTM assay	Phosphorylayion (Tyr-200)			16319926
TRPV1	SRC	Patch clamp			Activation	15084474
TRPV1	SRC	Patch clamp			Activation	16319926
TRPV1	TrkA	In vivo PTM assay	Phosphorylayion (Tyr-200)			16319926
TRPV1	TrkA	Patch clamp			Activation	16319926

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPV1 / interactors