TRPV2 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily V member 2
(TRPV2)
Official Symbol: TRPV2
provided by HGNC
Function:
Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
51393 |
Q9Y5S1 |
119520 |
Q9Y5S1 |
508 |
hsa:51393 |
606676 |
|||
|
Mouse
|
22368 |
Q9WTR1 |
204537 |
Q9WTR1 |
mmu:22368 |
|||||
|
Rat
|
29465 |
Q9WUD2 |
248108 |
Q9WUD2 |
rno:29465 |
|||||
PPI pairs:
Biological Process:
- GO:0045773
: positive regulation of axon extension - GO:0090280 : positive regulation of calcium ion import
- GO:0009408 : response to heat
- GO:0009266 : response to temperature stimulus
- GO:0007600 : sensory perception
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPV2 |
|
ACBD3 | Inference | Prediction | 15249591 | |||||
| TRPV2 |
|
AKAP5/AKAP150 | Speculation | Prediction | 18701070 | |||||
| TRPV2 |
|
Calmodulin | Inference | Prediction | 20686800 | |||||
| TRPV2 |
|
PKA | Inference | Prediction | 15249591 | |||||
| TRPV2 |
|
PKARIIơ | Inference | Prediction | 15249591 | |||||
| TRPV2 |
|
RGA/RAG1AP1 | Yeast two-hybrid | Rat | N-terminus | Rat | Brain | cDNA library | 14991772 | |
| TRPV2 |
|
SYNE1 | Yeast two-hybrid | Mouse | 1-167 | Human | Heart | cDNA library | 16875688 | |
| TRPV2 |
|
TRPV1 | Affinity purification-mass spectrometry | Not used | Mouse | Dorsal root ganglion tissue | Lysates | 24036102 | ||
| TRPV2 |
|
TRPV1 | Inference | Prediction | 15713749 | |||||
| TRPV2 |
|
UBC | Affinity purification-mass spectrometry | Not used as a bait | Human | HEK293T | Cell lystaes | 21890473 | ||
| TRPV2 |
|
UBC | Affinity purification-mass spectrometry | Not used as a bait | Human | ?HCT116 | Cell lystaes | 21906983 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPV2 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | Not specified | E. coli | 20926660 |
| TRPV2 |
|
SYNE1 | Fusion protein-pull down assay | Not used | Rat heart lysates | Human | 1-476 (in GSRP-56 Variant) | E. coli | 16875688 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV2 |
|
ACBD3 | Co-immunoprecipitation | RBL-2H3 | 15249591 | |||||
| TRPV2 |
|
AKAP5/AKAP150 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 18701070 | ||
| TRPV2 |
|
PKARIIơ | Co-immunoprecipitation | RBL-2H3 | 15249591 | |||||
| TRPV2 |
|
RGA/RAG1AP1 | Co-immunofluorescence staining | HEK293 | Rat | Full-length | Mouse | Full-length | 14991772 | |
| TRPV2 |
|
RGA/RAG1AP1 | Co-immunoprecipitation | RBL-2H3 | Not used | Mouse | Full-length | 14991772 | ||
| TRPV2 |
|
RGA/RAG1AP1 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Mouse | Full-length | 14991772 | |
| TRPV2 |
|
RGA/RAG1AP1 | Co-immunoprecipitation | RBL-2H3 | 15547947 | |||||
| TRPV2 |
|
RGA/RAG1AP1 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Human | Full-length | 15547947 | |
| TRPV2 |
|
TRPV1 | Co-immunofluorescence staining | Rat dorsal root ganglion tissue | 16115206 | |||||
| TRPV2 |
|
TRPV1 | Co-immunofluorescence staining | HEK293 | Rat | Full-length | Rat | Full-length | 16237318 | |
| TRPV2 |
|
TRPV1 | Co-immunofluorescence staining | Rat dorsal root ganglion section | 16237318 | |||||
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 16115206 | |
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | Rat brain cortical lysates | 16115206 | |||||
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 16237318 | |
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | Rat dorsal root ganglion derived cell line F11 | Rat | Full-length | Rat | Full-length | 16237318 | |
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | Rat dorsal root ganglion lysates | 16237318 | |||||
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | Rat spinal cord lysates | 16237318 | |||||
| TRPV2 |
|
TRPV1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Rat | Full-length | 15713749 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV2 |
|
Calmodulin | Fluorescence probe labeling | Human | 654-683 | Not specified | Not determined | 191 nM | 20686800 | |
| TRPV2 |
|
Calmodulin | Fusion protein-pull down assay | Rat | 684-753 | Not specified | Not determined | 20926660 | ||
| TRPV2 |
|
SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 (in GSRP-56 Variant) | 16875688 | ||
| TRPV2 |
|
SYNE1 | Fusion protein-pull down assay | Rat | Not determined | Human | 1-258 | 16875688 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPV2 |
|
PKA | In vitro PTM assay | Phosphorylation | 15249591 | |||||||
| TRPV2 |
|
RGA/RAG1AP1 | Cell surface biotinylation | Increase in plasma membrane level | 15547947 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)