TRP channels

TRPC
- TRPC1
- TRPC2
- TRPC3
- TRPC4
- TRPC5
- TRPC6
- TRPC7
TRPV
- TRPV1
- TRPV2
- TRPV3
- TRPV4
- TRPV5
- TRPV6
TRPM
- TRPM1
- TRPM2
- TRPM3
- TRPM4
- TRPM5
- TRPM6
- TRPM7
- TRPM8
TRPML
- TRPML1
- TRPML2
- TRPML3
TRPP
- TRPP1
- TRPP2
- TRPP3
TRPA
- TRPA1

» Citation

Nar2011 9c5ccb7544ea9ea0a4b3599ccec8bea610f3995142d5da2da0189fad9f699236

Plos2012 71954aaff6a4569630a779eb6502d687ff86630adfca3a1cc207b9836b808980

Pf2013 33c9f49bb4f61555bed5365c90448ebe56e96eb338f5692f7ef4b8a5db693247

TRPV4 / interactors

At a glance
In detail

Basic Information
External Database
PPI pairs (38)
Biological Process (17)
Disease (9)

Name: Transient receptor potential cation channel subfamily V member 4 (TRPV4)

Official Symbol: TRPV4 provided by HGNC

Function:

Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.

Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).

Species

External DB

Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984

Entrez Gene

UniprotKB

DIP

IntAct

MINT

BioGRID

STRING

IUPHAR-DB

KEGG

OMIM

Human

Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f

Mouse

Rat

PPI pairs:

80K-H	AKAP5/AKAP150	AQP-4	AQP-5	AT1aR	Bkca	Calmodulin	Caveolin-1
E-cadherin	FYN	HCK	IP3R3	ITCH/AIP4	LCK	LYN	MAP7
Myosin-10	Myosin-HIIA	NHERF4	OS-9	PACS-1	Pacsin1	Pacsin2	Pacsin3
RPS27A	Src	TMEM16A/ANO1	TRPC1	TRPP1	Tubulin	UBC	YES
ơ2-integrin	Ƣ-arrestin 1	Ƣ-arrestin 2	Ƣ-catenin	Ƣ-tubulin-5	ƣ-actin

Biological Process:

GO:0031532 : actin cytoskeleton reorganization
GO:0007015 : actin filament organization
GO:0070509 : calcium ion import
GO:0008219 : cell death
GO:0006884 : cell volume homeostasis
GO:0007043 : cell-cell junction assembly
GO:0071476 : cellular hypotonic response
GO:0043622 : cortical microtubule organization
GO:0007204 : elevation of cytosolic calcium ion concentration
GO:0042538 : hyperosmotic salinity response
GO:0046785 : microtubule polymerization
GO:0010977 : negative regulation of neuron projection development
GO:0007231 : osmosensory signaling pathway
GO:0031117 : positive regulation of microtubule depolymerization
GO:0047484 : regulation of response to osmotic stress
GO:0009612 : response to mechanical stimulus
GO:0030103 : vasopressin secretion

Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/

Disease:

OMIM:605427 : Brachyolmia type 3 (OMIM:113500 )
OMIM:605427 : Spondylometaphyseal dysplasia, Kozlowski type (OMIM:184252 )
OMIM:605427 : Metatropic dysplasia (OMIM:156530 )
OMIM:605427 : Hereditary motor and sensory neuropathy, type IIc (OMIM:606071 )
OMIM:605427 : Scapuloperoneal spinal muscular atrophy (OMIM:181405 )
OMIM:605427 : Sodium serum level QTL 1 (OMIM:613508 )
OMIM:605427 : Parastremmatic dwarfism (OMIM:168400 )
OMIM:605427 : SED, Maroteaux type (OMIM:184095 )
OMIM:605427 : Spinal muscular atrophy, distal, congenital nonprogressive (OMIM:600175 )

Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/

Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Screening
			Experimental screening					Non-experimental screening	Reference
			TRP channel construct		Interactor source
TRP channel	Interactor	Method	Species	Region	Species	Organ/tissue	Sample type
TRPV4	80K-H	Speculation						Prediction	19801576
TRPV4	AKAP5/AKAP150	Speculation						Prediction	18701070
TRPV4	AQP-4	Inference						Prediction	21262839
TRPV4	AQP-5	Inference						Prediction	16571723
TRPV4	AT1aR	Inference						Prediction	20650893
TRPV4	Bkca	Inference						Prediction	25139746
TRPV4	Calmodulin	Inference						Prediction	12724311
TRPV4	Caveolin-1	Inference						Prediction	20705915
TRPV4	E-cadherin	Inference						Prediction	20413591
TRPV4	FYN	Inference						Prediction	12538589
TRPV4	ƣ-actin	Inference						Prediction	22101010
TRPV4	Ƣ-actin	Inference						Prediction	19027987
TRPV4	Ƣ-arrestin 1	Inference						Prediction	20650893
TRPV4	Ƣ-arrestin 2	Affinity purification-mass spectrometry	Not used as a bait		Human		HEK293 lysates		17620599
TRPV4	Ƣ-catenin	Yeast two-hybrid	Mouse	N-terminus	Human	Keratinocyte	cDNA library		20413591
TRPV4	Ƣ-tubulin-5	Inference						Prediction	22309793
TRPV4	HCK	Inference						Prediction	12538589
TRPV4	IP3R3	Inference						Prediction	18378772
TRPV4	ITCH/AIP4	Inference						Prediction	17110928
TRPV4	LCK	Inference						Prediction	12538589
TRPV4	LYN	Inference						Prediction	12538589
TRPV4	MAP7	Yeast two-hybrid	Mouse	783-871	Mouse	Kidney	cDNA library		14517216
TRPV4	Myosin-10	Affinity purification-mass spectrometry	Mouse	Full-length	Human	HEK293	Cell lystaes		22492541
TRPV4	Myosin-HIIA	Affinity purification-mass spectrometry	Mouse	Full-length	Human	HEK293	Cell lystaes		22492541
TRPV4	NHERF4	Inference						Prediction	16565876
TRPV4	ơ2-integrin	Inference						Prediction	18234883
TRPV4	OS-9	Yeast two-hybrid	Mouse	61-467	Human	adult kidney	cDNA library		17932042
TRPV4	PACS-1	Inference						Prediction	15692563
TRPV4	Pacsin1	Yeast two-hybrid	Chicken	N-terminus	Chicken	Inner ear	cDNA library		16627472
TRPV4	Pacsin2	Inference						Prediction	16627472
TRPV4	Pacsin3	Yeast two-hybrid	Chicken	N-terminus	Chicken	Inner ear	cDNA library		16627472
TRPV4	RPS27A	Affinity purification-mass spectrometry	Mouse	Full-length	Human	HEK293	Cell lystaes		22492541
TRPV4	SRC	Inference						Prediction	12538589
TRPV4	TMEM16A/ANO1	Inference						Prediction	24509911
TRPV4	TRPC1	Inference						Prediction	20705915
TRPV4	TRPP1	Inference						Prediction	18695040
TRPV4	UBC	Inference						Prediction	17110928
TRPV4	YES	Inference						Prediction	12538589

(

Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vitro validation
			Assay with recombinant proteins						Reference
			TRP channel construct			Interactor construct
TRP channel	Interactor	Method	Species	Region	Expression system	Species	Region	Expression system
TRPV4	Calmodulin	Fusion protein-pull down assay	Mouse	Full-length	HEK293 lysates	Not specified	Not specified	In vitro translation	22492541
TRPV4	Calmodulin	Fusion protein-pull down assay	Human	718-871	E. coli	Not used		HEK293T lysates	21837266
TRPV4	Calmodulin	Fusion protein-pull down assay	Human	C-terminus	E. coli	Not specified	Full-length	Not specified	12724311
TRPV4	ƣ-actin	Fusion protein-pull down assay	Mouse	Full-length	E. coli	Not used		HEK293 lysates	22101010
TRPV4	MAP7	Fusion protein-pull down assay	Mouse	Full-length	HEK293 lysates	Mouse	Full-length	In vitro translation	14517216
TRPV4	NHERF4	Fusion protein-pull down assay	Mouse	C-terminus	E. coli	Mouse	Full-length	In vitro translation	16565876
TRPV4	Tubulin	Fusion protein-pull down assay	Mouse	Full-length	E. coli	Not used		HEK293 lysates	22101010

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Validation: In vivo validation
			Assay with endogenous proteins	Assay with overexpressed proteins					Reference
			Cell or tissue	Cell or tissue	TRP channel construct		Interactor construct
TRP channel	Interactor	Method	Cell or tissue	Cell or tissue	Species	Region	Species	Region
TRPV4	80K-H	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Human	Full-length	19801576
TRPV4	AKAP5/AKAP150	Co-immunofluorescence staining	Rat cerebral artery						24778429
TRPV4	AKAP5/AKAP150	Co-immunoprecipitation		HEK293	Human	Full-length	Not used		18701070
TRPV4	AQP-4	Co-immunofluorescence staining	Adult rat brain section						21262839
TRPV4	AQP-4	Co-immunoprecipitation	Adult rat brain lysates						21262839
TRPV4	AQP-4	Co-immunoprecipitation		DI TNC1	Human	Full-length	Rat	Full-length	21262839
TRPV4	AQP-5	Co-immunoprecipitation	Mouse submandibular gland cell						16571723
TRPV4	AQP-5	Co-immunoprecipitation	HSG cell						16571723
TRPV4	AT1aR	Co-immunofluorescence staining		Rat vascular smooth muscle cell	Human	Full-length	Human	Full-length	20650893
TRPV4	AT1aR	Co-immunoprecipitation		Rat vascular smooth muscle cell	Not used		Human	Full-length	20650893
TRPV4	AT1aR	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	20650893
TRPV4	Bkca	Co-immunoprecipitation	Porcine coronary artery						25139746
TRPV4	Caveolin-1	Co-immunofluorescence staining		HUVEC	Human	Full-length	Mouse	Full-length	20705915
TRPV4	E-cadherin	Co-immunoprecipitation	MDCK						20413591
TRPV4	E-cadherin	Co-immunoprecipitation	HEK						22374181
TRPV4	FYN	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		12538589
TRPV4	Ƣ-actin	Co-immunofluorescence staining		HaCaT	Human	Full-length	Not used		19027987
TRPV4	ƣ-actin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		22101010
TRPV4	Ƣ-actin	Fluorescence resonance energy transfer		CHO	Human	Full-length	Human	Full-length	19027987
TRPV4	Ƣ-arrestin 1	Co-immunoprecipitation	Rat vascular smooth muscle cell						20650893
TRPV4	Ƣ-arrestin 1	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	20650893
TRPV4	Ƣ-catenin	Co-immunoprecipitation	MDCK						20413591
TRPV4	Ƣ-catenin	Co-immunoprecipitation		COS-7	Mouse	Full-length	Not specified	Not specified	20413591
TRPV4	Ƣ-catenin	Co-immunoprecipitation	HEK						22374181
TRPV4	Ƣ-tubulin-5	Co-immunofluorescence staining		HEK293	Rat	Full-length	Rat	Full-length	22309793
TRPV4	Ƣ-tubulin-5	Co-immunoprecipitation		HEK293	Rat	Full-length	Rat	Full-length	22309793
TRPV4	HCK	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		12538589
TRPV4	IP3R3	Co-immunoprecipitation		HeLa	Human	Full-length	Rat	Full-length	18378772
TRPV4	IP3R3	Co-immunoprecipitation		HEK293	Human	Full-length	Rat	Full-length	18826956
TRPV4	ITCH/AIP4	Co-immunofluorescence staining		HEK293T	Mouse	Full-length	Mouse	Full-length	17110928
TRPV4	ITCH/AIP4	Co-immunoprecipitation		HEK293	Human	Full-length	Human	Full-length	20650893
TRPV4	ITCH/AIP4	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Mouse	Full-length	17110928
TRPV4	LCK	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		12538589
TRPV4	LYN	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Not used		12538589
TRPV4	LYN	Co-immunoprecipitation	Rat dorsal root ganglion neuron						18234883
TRPV4	LYN	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	12538589
TRPV4	MAP7	Co-immunofluorescence staining	Mouse lung section						14517216
TRPV4	MAP7	Co-immunofluorescence staining	Mouse kidney section						14517216
TRPV4	MAP7	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Mouse	Full-length	14517216
TRPV4	MAP7	Co-immunoprecipitation	Mouse renal tissue						14517216
TRPV4	MAP7	Co-immunoprecipitation		HEK293	Mouse	785-808	Mouse	Full-length	14517216
TRPV4	Myosin-10	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Not used		22492541
TRPV4	Myosin-HIIA	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Not used		22492541
TRPV4	ơ2-integrin	Co-immunoprecipitation	Rat dorsal root ganglion neuron						18234883
TRPV4	OS-9	Co-immunofluorescence staining		SJSA-1	Mouse	Full-length	Not used		17932042
TRPV4	OS-9	Co-immunoprecipitation		HEK293T	Mouse	61-467	Human	430-667	17932042
TRPV4	PACS-1	Co-immunoprecipitation		HEK293T	Human	Full-length	Human	Full-length	15692563
TRPV4	Pacsin1	Co-immunoprecipitation		HEK293	Rat	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin2	Co-immunofluorescence staining		HEK293	Rat	Full-length	Mouse	Full-length	18174177
TRPV4	Pacsin2	Co-immunoprecipitation		HEK293	Rat	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin2	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin3	Co-immunofluorescence staining	Mouse kidney renal tubule						16627472
TRPV4	Pacsin3	Co-immunofluorescence staining		NIH-3T3	Rat	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin3	Co-immunofluorescence staining		HEK293	Rat	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin3	Co-immunofluorescence staining		HEK293	Rat	Full-length	Mouse	Full-length	18174177
TRPV4	Pacsin3	Co-immunoprecipitation	Mouse kidney tissue						18174177
TRPV4	Pacsin3	Co-immunoprecipitation		HEK293	Rat	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin3	Co-immunoprecipitation		HEK293	Mouse	Full-length	Mouse	Full-length	16627472
TRPV4	Pacsin3	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Mouse	Full-length	23690576
TRPV4	SRC	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		12538589
TRPV4	TMEM16A/ANO1	Co-immunoprecipitation		HEK293T	Mouse	Full-length	Mouse	Full-length	24509911
TRPV4	TRPC1	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Human	Full-length	20093626
TRPV4	TRPC1	Co-immunofluorescence staining	Rat mesenteric artery endothelial cell lysates						20093626
TRPV4	TRPC1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	20093626
TRPV4	TRPC1	Co-immunoprecipitation	Rat mesenteric artery endothelial cell lysates						20093626
TRPV4	TRPC1	Co-immunoprecipitation		Rat mesenteric artery endothelial cell	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPC1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPC1	Co-immunoprecipitation	Porcine coronary artery						25139746
TRPV4	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	20093626
TRPV4	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	20705915
TRPV4	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	21871867
TRPV4	TRPC1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPP1	Co-immunofluorescence staining	MDCK						18695040
TRPV4	TRPP1	Co-immunofluorescence staining		HEK293	Mouse	Full-length	Human	Full-length	22518003
TRPV4	TRPP1	Co-immunofluorescence staining		CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD)	Mouse	Full-length	Human	Full-length	22518003
TRPV4	TRPP1	Co-immunofluorescence staining	Mouse orpk collecting duct cell line						23977387
TRPV4	TRPP1	Co-immunoprecipitation		HEK293 tsA 201	Mouse	Full-length	Human	Full-length	20682256
TRPV4	TRPP1	Co-immunoprecipitation		Rat mesenteric artery endothelial cell	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPP1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPP1	Co-immunoprecipitation		HEK293	Mouse	C-terminus	Human	C-terminus	18695040
TRPV4	TRPP1	Co-immunoprecipitation		HEK293	Mouse	Full-length	Human	Full-length	22518003
TRPV4	TRPP1	Co-immunoprecipitation		CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD)	Mouse	Full-length	Human	Full-length	22518003
TRPV4	TRPP1	Co-immunoprecipitation	Mouse inner medullary collecting duct cell						23977387
TRPV4	TRPP1	Co-immunoprecipitation	Mouse orpk collecting duct cell line						23977387
TRPV4	TRPP1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	25114176
TRPV4	TRPP1	Fluorescence resonance energy transfer		HEK293	Mouse	Full-length	Human	Full-length	18695040
TRPV4	Tubulin	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		22101010
TRPV4	YES	Co-immunoprecipitation		HEK293	Mouse	Full-length	Not used		12538589

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Characterization
			Binding region mapping				Stoichiometry	Affinity (Kd)	Reference
			TRP channel		Interactor
TRP channel	Interactor	Method	Species	Region	Species	Region
TRPV4	Calmodulin	Fusion protein-pull down assay	Rat	ARD domain	Not specified	Not determined			20926660
TRPV4	Calmodulin	Fusion protein-pull down assay	Chicken	132-383	Not specified	Not determined			19864432
TRPV4	Calmodulin	Fusion protein-pull down assay	Human	718-871	Human	Not determined			21837266
TRPV4	Calmodulin	Fusion protein-pull down assay	Human	812-831	Not specified	Not determined			12724311
TRPV4	FYN	Co-immunoprecipitation	Mouse	Not determined	Mouse	145-247			12538589
TRPV4	ƣ-actin	Fusion protein-pull down assay	Mouse	718-871	Human	Not determined			22101010
TRPV4	Ƣ-catenin	Co-immunoprecipitation	Mouse	Not determined	Not specified	Not determined			20413591
TRPV4	IP3R3	Co-immunoprecipitation	Human	812-831	Rat	Not determined			18826956
TRPV4	OS-9	Co-immunoprecipitation	Mouse	438-468	Human	430-667			17932042
TRPV4	PACS-1	Co-immunoprecipitation	Human	1-486	Human	Not determined			15692563
TRPV4	Pacsin3	Co-immunoprecipitation	Mouse	132-144	Mouse	368-424			16627472
TRPV4	Pacsin3	Co-immunoprecipitation	Rat	132-144	Mouse	368-424			16627472
TRPV4	TRPC1	Fluorescence resonance energy transfer					2:2		21871867
TRPV4	TRPP1	Atomic force microscopy(AFM) analysis					2:2		20682256
TRPV4	Tubulin	Fusion protein-pull down assay	Mouse	718-871	Human	Not determined			22101010

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

Screening

Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b

Functional consequence

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Functional consequence
TRP channel	Interactor	Method	Post-translational modification	Subcellular trafficking	Activity	Reference
TRPV4	AKAP5/AKAP150	Patch clamp			Activation	25005230
TRPV4	Calmodulin	Patch clamp			Activation	12724311
TRPV4	FYN	Calcium measurement			Activation	12538589
TRPV4	FYN	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589
TRPV4	HCK	Calcium measurement			Activation	12538589
TRPV4	HCK	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589
TRPV4	IP3R3	Calcium measurement			Activation	18378772
TRPV4	IP3R3	Calcium measurement			Activation	18826956
TRPV4	IP3R3	Patch clamp			Activation	18378772
TRPV4	IP3R3	Patch clamp			Activation	18826956
TRPV4	ITCH/AIP4	Co-immunofluorescence staining		Decrease in plasma membrane level		17110928
TRPV4	LCK	Calcium measurement			Activation	12538589
TRPV4	LCK	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589
TRPV4	LYN	Calcium measurement			Activation	12538589
TRPV4	LYN	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589
TRPV4	MAP7	Cell surface biotinylation		Increase in plasma membrane level		14517216
TRPV4	MAP7	Patch clamp			Activation	14517216
TRPV4	OS-9	Cell surface biotinylation		Retain in the endoplasmic reticulum		17932042
TRPV4	Pacsin3	Co-immunofluorescence staining		Increase in plasma membrane level		16627472
TRPV4	Pacsin3	Patch clamp			Inhibition	18174177
TRPV4	SRC	Calcium measurement			Activation	12538589
TRPV4	SRC	Calcium measurement			Activation	19033444
TRPV4	SRC	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589
TRPV4	SRC	In vivo PTM assay	Phosphorylation (Tyr-110)			19033444
TRPV4	SRC	In vivo PTM assay	Phosphorylation (Tyr-805)			19033444
TRPV4	TRPC1	Calcium measurement			Activation	20093626
TRPV4	TRPC1	Calcium measurement			Activation	20705915
TRPV4	TRPC1	Cell surface biotinylation		Increase in plasma membrane level		20705915
TRPV4	TRPC1	Co-immunofluorescence staining		Increase in plasma membrane level		20705915
TRPV4	TRPC1	Patch clamp			New channel creation	25114176
TRPV4	TRPP1	Calcium measurement			New channel creation	22518003
TRPV4	TRPP1	Patch clamp			New channel creation	25114176
TRPV4	TRPP1	Patch clamp			New channel creation	18695040
TRPV4	UBC	In vivo PTM assay	Ubiquitination			17110928
TRPV4	UBC	In vivo PTM assay	Ubiquitination			17932042
TRPV4	YES	Calcium measurement			Activation	12538589
TRPV4	YES	In vitro PTM assay	Phosphorylation (Tyr-273)			12538589

(

: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

TRIP Database

TRPV4 / interactors