TRPV6 / interactors
- At a glance
- In detail
Name: Transient receptor potential cation channel subfamily V member 6
(TRPV6)
Official Symbol: TRPV6
provided by HGNC
Function:
Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
| Species |
External DB
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
|
55503 |
Q9H1D0 |
DIP-43888N |
MINT-1472896 |
120683 |
Q9H1D0 |
512 |
hsa:55503 |
606680 |
|
|
Mouse
|
64177 |
Q91WD2 |
211037 |
Q91WD2 |
mmu:64177 |
|||||
|
Rat
|
114246 |
Q9R186 |
DIP-47769N |
UNK-5555281 |
250323 |
Q9R186 |
rno:114246 |
|||
PPI pairs:
Biological Process:
- GO:0017158
: regulation of calcium ion-dependent exocytosis
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
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|---|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPV6 |
|
BSPRY | Yeast two-hybrid | Mouse | 597-601 | Mouse | Kidney | cDNA library | 16380433 | |
| TRPV6 |
|
Calbindin-D28K | Inference | Prediction | 16763551 | |||||
| TRPV6 |
|
Calmodulin | Inference | Prediction | 11248124 | |||||
| TRPV6 |
|
Cyclophilin B | Affinity purification-mass spectrometry | Human | 1-725 | Human | Placenta | Microsomal membrane fraction | 18445599 | |
| TRPV6 |
|
FYN | Inference | Prediction | 17197020 | |||||
| TRPV6 |
|
I-MFA | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified | 16189514 | |
| TRPV6 |
|
Klotho | Inference | Prediction | 16239475 | |||||
| TRPV6 |
|
NHERF4 | Yeast two-hybrid | Mouse | 575-727 | Mouse | Kidney | cDNA library | 17645868 | |
| TRPV6 |
|
NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library | 16565876 | |
| TRPV6 |
|
Nipsnap1 | Inference | Prediction | 18392847 | |||||
| TRPV6 |
|
Numb | Inference | Prediction | 23140583 | |||||
| TRPV6 |
|
PTEN | Inference | Prediction | 24704446 | |||||
| TRPV6 |
|
PTP1B | Inference | Prediction | 15894168 | |||||
| TRPV6 |
|
Rab11a | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library | 16354700 | |
| TRPV6 |
|
RGS2 | Yeast two-hybrid | Mouse | 1-326 | Mouse | Kidney | cDNA library | 16895908 | |
| TRPV6 |
|
RyR1 | Affinity purification-mass spectrometry | Not used as a bait | Rabbit | Fast-twitch muscle | Triad vesicles | 18215135 | ||
| TRPV6 |
|
S100A10 | Inference | Prediction | 12660155 | |||||
| TRPV6 |
|
S100A10 | Yeast two-hybrid | Human | Not specified | Human | Not specified | Not specified | 16189514 | |
| TRPV6 |
|
SRC | Inference | Prediction | 17197020 | |||||
| TRPV6 |
|
TRPC1 | Inference | Prediction | 22932896 | |||||
| TRPV6 |
|
TRPML3 | Inference | Prediction | 23469151 | |||||
| TRPV6 |
|
TRPV5 | Inference | Prediction | 12574114 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPV6 |
|
BSPRY | Fusion protein-pull down assay | Mouse | 597-601 | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 16380433 |
| TRPV6 |
|
BSPRY | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Mouse | Full-length | E. coli | 16380433 |
| TRPV6 |
|
Calbindin-D28K | Fusion protein-pull down assay | Mouse | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551 |
| TRPV6 |
|
Calbindin-D28K | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Not specified | Full-length | In vitro translation | 16763551 |
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | 584-725 | In vitro translation | Bovine | Full-length | E. coli | 11248124 |
| TRPV6 |
|
NHERF4 | Fusion protein-pull down assay | Mouse | 575-727 | E. coli | Mouse | Full-length | In vitro translation | 17645868 |
| TRPV6 |
|
NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876 |
| TRPV6 |
|
Nipsnap1 | Fusion protein-pull down assay | Mouse | 577-607 | E. coli | Not specified | Full-length | E. coli | 18392847 |
| TRPV6 |
|
PTP1B | Yeast two-hybrid | Rat | 1-326 | Yeast | Human | Full-length | Yeast | 17197020 |
| TRPV6 |
|
Rab11a | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | In vitro translation | 16354700 |
| TRPV6 |
|
RGS2 | Fusion protein-pull down assay | Mouse | Full-length | HEK293 lysates | Human | Full-length | E. coli | 16895908 |
| TRPV6 |
|
S100A10 | Fusion protein-pull down assay | Mouse | Full-length | E. coli | Mouse | Full-length | Xenopus oocyte lysates | 12660155 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV6 |
|
Calmodulin | Co-immunoprecipitation | Xenopus oocyte | Rabbit | Full-length | Not specified | Full-length | 15123711 | |
| TRPV6 |
|
Calmodulin | Fluorescence resonance energy transfer | HEK293 | Rat | Full-length | Not specified | Full-length | 16959851 | |
| TRPV6 |
|
Cyclophilin B | Co-immunofluorescence staining | Xenopus oocyte | Human | Full-length | Human | Full-length | 18445599 | |
| TRPV6 |
|
Cyclophilin B | Co-immunofluorescence staining | Human placenta | 18445599 | |||||
| TRPV6 |
|
Cyclophilin B | Co-immunoprecipitation | Human placenta | 18445599 | |||||
| TRPV6 |
|
NHERF4 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876 | |
| TRPV6 |
|
Nipsnap1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not specified | Full-length | 18392847 | |
| TRPV6 |
|
Numb | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 24704446 | |
| TRPV6 |
|
Numb | Co-immunoprecipitation | Du145 | 24704446 | |||||
| TRPV6 |
|
Numb | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 23140583 | |
| TRPV6 |
|
PTEN | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 24704446 | |
| TRPV6 |
|
PTEN | Co-immunoprecipitation | Du145 | 24704446 | |||||
| TRPV6 |
|
PTP1B | Biomolecular fluorescence complementation | HEK293 | Rat | Full-length | Human | Full-length | 15894168 | |
| TRPV6 |
|
PTP1B | Biomolecular fluorescence complementation | HEK293 | Rat | Full-length | Human | Full-length | 17197020 | |
| TRPV6 |
|
Rab11a | Co-immunofluorescence staining | Mouse kidney section | 16354700 | |||||
| TRPV6 |
|
Rab11a | Co-immunofluorescence staining | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16354700 | |
| TRPV6 |
|
S100A10 | Co-immunofluorescence staining | Rat duodenum tissue | 12660155 | |||||
| TRPV6 |
|
S100A10 | Co-immunoprecipitation | Caco-2 cell | 18187190 | |||||
| TRPV6 |
|
S100A10 | Co-immunoprecipitation | Human 16HBE14o- cell | 18187190 | |||||
| TRPV6 |
|
SRC | Co-immunoprecipitation | HEK293 | Rat | Full-length | Human | Full-length | 17197020 | |
| TRPV6 |
|
TRPC1 | Co-immunoprecipitation | HEK293 | Rat | Full-length | Human | Full-length | 22932896 | |
| TRPV6 |
|
TRPC1 | Co-immunoprecipitation | LNCaP | 25172921 | |||||
| TRPV6 |
|
TRPC1 | Fluorescence resonance energy transfer | HEK293 | Rat | Full-length | Human | Full-length | 22932896 | |
| TRPV6 |
|
TRPML3 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Not specified | Full-length | 23469151 | |
| TRPV6 |
|
TRPV5 | Co-immunofluorescence staining | Mouse kidney section | 12574114 | |||||
| TRPV6 |
|
TRPV5 | Co-immunoprecipitation | Human myeloid leukemia K562 cell | 19295174 | |||||
| TRPV6 |
|
TRPV5 | Co-immunoprecipitation | Xenopus oocyte | Mouse | Full-length | Rat | Full-length | 12574114 | |
| TRPV6 |
|
TRPV5 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749 | |
| TRPV6 |
|
TRPV5 | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Mouse | Full-length | 23469151 | |
| TRPV6 |
|
TRPV5 | Fluorescence resonance energy transfer | HEK293 | Mouse | Full-length | Mouse | Full-length | 15713749 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPV6 |
|
Calmodulin | Fluorescence probe labeling | 1:1 | 43 nM | 12765696 | ||||
| TRPV6 |
|
Calmodulin | Fluorescence probe labeling | 65 nM | 11248124 | |||||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 327-577 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 88-97 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Rabbit | 643-656 | Not specified | Not determined | 15123711 | ||
| TRPV6 |
|
Calmodulin | Fusion protein-pull down assay | Human | 691-711 | Bovine | Not determined | 11248124 | ||
| TRPV6 |
|
NHERF4 | Fusion protein-pull down assay | Mouse | 724-727 | Mouse | Not determined | 17645868 | ||
| TRPV6 |
|
Nipsnap1 | Fusion protein-pull down assay | Mouse | 83-118 | Not specified | Not determined | 18392847 | ||
| TRPV6 |
|
Numb | Co-immunoprecipitation | Human | 716 | Human | 434 | 23140583 | ||
| TRPV6 |
|
PTP1B | Biomolecular fluorescence complementation | Rat | 1-191 | Human | Not determined | 17197020 | ||
| TRPV6 |
|
S100A10 | Fusion protein-pull down assay | Mouse | 597-601 | Mouse | Not determined | 12660155 | ||
| TRPV6 |
|
TRPC1 | Fluorescence resonance energy transfer | Rat | 1-198 | Human | 1-193 | 22932896 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
| TRPV6 |
|
Calmodulin | Calcium measurement | Inhibition | 16959851 | |||||||
| TRPV6 |
|
Calmodulin | Patch clamp | Inhibition | 15123711 | |||||||
| TRPV6 |
|
Calmodulin | Patch clamp | Inhibition | 11248124 | |||||||
| TRPV6 |
|
Cyclophilin B | Calcium measurement | Activation | 18445599 | |||||||
| TRPV6 |
|
FYN | In vitro PTM assay | Phosphorylation | 17197020 | |||||||
| TRPV6 |
|
Klotho | Calcium measurement | Activation | 18495742 | |||||||
| TRPV6 |
|
Klotho | In vitro PTM assay | Glycosylation (Asn-357) | 16239475 | |||||||
| TRPV6 |
|
Klotho | Patch clamp | Activation | 16239475 | |||||||
| TRPV6 |
|
NHERF4 | Patch clamp | Activation | 17645868 | |||||||
| TRPV6 |
|
Nipsnap1 | Patch clamp | Inhibition | 18392847 | |||||||
| TRPV6 |
|
Numb | Calcium measurement | Inhibition | 23140583 | |||||||
| TRPV6 |
|
PTP1B | Calcium measurement | Inhibition | 15894168 | |||||||
| TRPV6 |
|
PTP1B | Calcium measurement | Inhibition | 17197020 | |||||||
| TRPV6 |
|
PTP1B | In vitro PTM assay | Dephosphorylation | 15894168 | |||||||
| TRPV6 |
|
PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-162) | 17197020 | |||||||
| TRPV6 |
|
PTP1B | In vivo PTM assay | Dephosphorylation (Tyr-161) | 17197020 | |||||||
| TRPV6 |
|
Rab11a | Calcium measurement | Activation | 16354700 | |||||||
| TRPV6 |
|
Rab11a | Co-immunofluorescence staining | Increase in plasma membrane level | 16354700 | |||||||
| TRPV6 |
|
RGS2 | Patch clamp | Inhibition | 16895908 | |||||||
| TRPV6 |
|
S100A10 | Co-immunofluorescence staining | Increase in plasma membrane level | 12660155 | |||||||
| TRPV6 |
|
S100A10 | Patch clamp | Activation | 12660155 | |||||||
| TRPV6 |
|
SRC | In vitro PTM assay | Phosphorylation | 17197020 | |||||||
| TRPV6 |
|
SRC | In vivo PTM assay | Phosphorylation (Tyr-162) | 17197020 | |||||||
| TRPV6 |
|
SRC | In vivo PTM assay | Phosphorylation (Tyr-161) | 17197020 | |||||||
| TRPV6 |
|
TRPC1 | Cell surface biotinylation | Retain in the endoplasmic reticulum | 22932896 | |||||||
| TRPV6 |
|
TRPC1 | Patch clamp | Inhibition | 22932896 | |||||||
| TRPV6 |
|
TRPV5 | Patch clamp | New channel creation | 12574114 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)